Anti-β-Actin Monoclonal antibody抗體

英文名稱 Anti-β-Actin Monoclonal antibody

宿主 Mouse

別名 β-Actin

應用 WB IHC IF

稀釋比例 WB 1:3000-8000. IHC 1:100-300. IF 1:100-200.

交叉反應 Human Mouse Rat Monkey Chicken Rabbit Hamster

蛋白分子量 43kDa

Gene ID 60

保存 Store at -20°C. Avoid freeze / thaw cycles.

儲存液 Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.

純化方法 Affinity purification

亞型 IgG1

免疫原 Recombinant protein of human β-Actin

性狀 液體

Public Immunogen Range Recombinant protein of human β-Actin

Subcellular Locations Nucleus Cytoskeleton

Swiss Prot P60709

克隆類型 Monoclonal Antibody

背景資料 β-Actin?is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against?β-Actin?are useful as loading controls for Western Blotting. However it should be noted that levels of?β-Actin?may not be stable in certain cells. For example, expression of?β-Actin?in adipose tissue is very low and therefore?β-Actin?should not be used as loading control for these tissues.

參考文獻：

《Epigenetically upregulated oncoprotein PLCE1 drives esophageal carcinoma angiogenesis and proliferation via activating the PI-PLCε-NF-κB signaling pathway and VEGF-C/ Bcl-2 expression》 作者:Yunzhao Chen, Dandan Wang, Hao Peng, Xi Chen, Xueping Han, Jie Yu, Wenjie Wang, Lirong Liang, Zheng Liu, Yi Zheng, Jianming Hu, Lan Yang, Jun Li, Hong Zhou, Xiaobin Cui and Feng Li 期刊:Molecular Cancer 影響因子:4.257 PMID:30609930

《CRISPR-Cas9 Mediated RNase L Knockout Regulates Cellular Function of PK-15 Cells and Increases PRV Replication》 作者:Chao Sui, Dandan Jiang,Xiangju Wu, Xiaoyan Cong, Feng Li, Yingli Shang, Jinqiu Wang,Sidang Liu, Hu Shan, Jing Qiand Yijun Du 期刊:BioMed Research International 影響因子:2.197 PMID:30941371

《Endoplasmic Reticulum Stress Induces Macrophages to Produce IL-1β During Mycobacterium bovis Infection via a Positive Feedback Loop Between Mitochondrial Damage and Inflammasome Activation》 作者:Yi Liao,Tariq Hussain, Chunfa Liu, Yongyong Cui, Jie Wang, Jiao Yao,Hehua Chen, Yinjuan Song, Naveed Sabir, Mazhar Hussain,Deming Zhaoand Xiangmei Zhou 期刊:Frontiers in Immunology 影響因子:4.716 PMID:30846986

《Effect of artemisinin on proliferation and apoptosis-related protein expression in vivo and in vitro》 作者:JiajunChen,LeiZhang,MiaoHao 期刊:Saudi Journal of Biological Sciences 影響因子:2.82 PMID:30505200

《High expression of active ATF6 aggravates endoplasmic reticulum stress?induced vascular endothelial cell apoptosis through the mitochondrial apoptotic pathway》 作者:Jingyong Huang ,Li Wan, Heping Lu ,Xiaoqiang Li 期刊:Molecular Medicine Reports 影響因子:1.851 PMID:29512699

《Activation of Prodrugs by NIR-Triggered Release of Exogenous Enzymes for Locoregional Chemo-photothermal Therapy》 作者:Li Cheng , Fengrong Zhang , Shunhao Wang,  Xueting Pan , Sichong Han,  Shuang Liu , Junjie Ma ,Hongyu Wang,  Prof. Heyun Shen,  Prof. Huiyu Liu,  Prof. Qipeng Yuan 期刊:Angew. Chem. Int. Ed. 影響因子:12.102 PMID:30964594

Anti-β-Actin Monoclonal antibody抗體