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【ACE 色譜柱】甾體類藥物UHPLC-UV分析比較
Steroids UHPLC-UV Analysis and Comparison
Conditions
Column: ACE Excel 2 CN-ES
ACE Excel 2 C18
ACE Excel 2 CN
Dimensions: 50 x 2.1 mm
Part Number:
EXL -1013-0502U,
EXL -101-0502U,
EXL -104-0502U
Mobile Phase:
A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeCN
Gradient:
Time(mins) | %B |
0.0 | 25 |
10.0 | 80 |
10.5 | 80 |
11.0 | 25 |
Flow Rate: 0.4 mL/min
Temperature: 40 °C
Detection: UV, 260 nm
Analytes
1. Cortisone
2. Corticosterone
3. 11α-Hydroxyprogesterone
4. Cortisone-21-acetate
5. 11-Ketoprogesterone
6. β-Estradiol
7. 17α-Estradiol
8. 17α-Ethinylestradiol
9. Estrone