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【ACE Excel C18-Amide色譜柱】提高極性選擇性的甾體分離
Steroids Separation using Enhanced Polar Selectivity
Conditions
Column: ACE Excel 3 C18-Amide
Dimensions: 50 x 2.1 mm
Part Number: EXL -1112-0502U
Mobile Phase:
A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeCN
Gradient:
Time(mins) | %B |
0 | 25 |
10 | 80 |
Flow Rate: 0.4 mL/min
Temperature: 20 °C
Detection: UV, 260 nm
Analytes
1. Prednisone
2. Prednisolone
3. Estriol
4. Corticosterone
5. 11α-Hydroxyprogesterone
6. 11-Ketoprogesterone
7. 21-Hydroxyprogesterone
8. β-Estradiol
9. 17α-Estradiol
10. 17α-Ethinylestradiol