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【ACE Excel C18-AR色譜柱】LC-MS/MS測定甲狀腺激素(一)色譜分析
Thyroid Hormones by LC-MS/MS (I)
Conditions
Column: ACE Excel 2 C18-AR
Dimensions: 100 x 2.1 mm
Part Number: EXL -109-1002U
Mobile Phase:
A: 2 mM ammonium acetate, 0.1% formic acid in H2O
B: 2 mM ammonium acetate, 0.1% formic acid in MeOH
Gradient:
Time(mins) | %B |
0.0 | 60 |
3.0 | 77 |
3.1 | 60 |
Flow Rate: 0.4 mL/min
Injection: 10 μL
Temperature: 40 °C
Detection: XEVO TQS triple quad MS
Desolvation temperature: 500 °C
Ion source temperature: 150 °C Positive mode ESI, MRM
Sample: Serum samples extracted using Biotage EVOLUTE EXPRESS AX methodology
Analytes
1. T3 (Tri-iodothyronine)
2. rT3 (Reversed tri-iodothyronine)
3. T4 (Thyroxine)