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【ACE Excel C18-PFP色譜柱】生育酚混合物分離
Tocopherols Mixture Separation
Conditions
Column: ACE Excel 3 C18-PFP
Dimensions: 150 x 4.6 mm
Part Number: EXL -1110-1546U
Mobile Phase: A: 0.1% H3PO4/MeCN (1:3 v/v)
B: MeCN
Gradient:
Time(mins) | %B |
0.00 | 0 |
0.10 | 0 |
0.11 | 80 |
8.00 | 80 |
8.01 | 100 |
12.00 | 100 |
12.01 | 0 |
14.00 | 0 |
Flow Rate: 1.2 mL/min
Injection: 10 μL
Temperature: 40 °C
Detection: UV, 285 nm
Analytes
1. γ-Tocopherol
2. β-Tocopherol
3. δ-Tocopherol
4. α-Tocopherol
5. α-Tocopherol acetate