描述
兔多克隆抗體to Collagen IV
宿主
Rabbit
特異性
ab6586 is designed to bind specifically to NATIVE collagen epitopes composed of multiple subunit strands. Negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
經(jīng)測試應(yīng)用
適用于: IHC-Pmore details
種屬反應(yīng)性
與反應(yīng): Human
預(yù)測可用于: Cow
免疫原
Full length native protein (purified) corresponding to Collagen IV. Collagen Type IV from human and bovine placenta. The immunogen maintains the native conformation of the protein.
陽性對照
IHC-P: Human kidney and liver tissue.
常規(guī)說明
There are other recombinant monoclonal options, such as Recombinant Anti-Collagen IV antibody.Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor
488 (ab150077).See other anti-rabbit secondary antibodies that can be used with this antibody.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
形式
Liquid
存放說明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存儲溶液
Preservative: 0.01% Sodium azide
Constituents: 0.8766% Sodium chloride, 0.424% Potassium phosphate
濃度
100 µg 濃度為 1 mg/ml
純度
Immunogen affinity purified
純化說明
Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
Primary antibody說明
This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
克隆
多克隆
同種型
IgG
