CD34 Molecule （CD34） 抗體 （PE） 返回列表頁

CD34 Molecule (CD34) 抗體 (PE)

抗原
CD34 Molecule (CD34)
別名 AU040960
適用
 其他選擇Murine
宿主
 其他選擇Rat
克隆類型 (克隆位點)
單克隆 (MEC14-7)
標記
 其他選擇PE
應(yīng)用范圍
 其他選擇Flow Cytometry (FACS)
[Pubmed] 5 references available
規(guī)格 100 tests
發(fā)貨至 中國 (更改)
發(fā)貨時間 29至35個工作日

CD34 Molecule (CD34) 抗體 (PE)

產(chǎn)品細節(jié)

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克隆位點 MEC14-7
亞型 IgG2a
特異性 This antibody recognises the Murine CD34 cell surface antigen which is expressed by endothelial cells and by haematopoietic stem cells. This antibody recognises a Neuraminidase sensitive epitope. As in the Human system, CD34 antibodies in the Mouse demonstrate slightly different staining patterns depending on their fine specificity. Clone MEC14.7 appears to recognise a subset of the stem cell population recognised by clone RAM34, and it is thought that this is due to differences in the epitope recognised by the two antibodies.
交叉反應(yīng) （詳細） Species reactivity (tested):Mouse.
純化方法 Affinity Chromatography on Protein G.

目標詳細情況

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別名 CD34
背景 The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker
基因ID 12490
NCBI Accession NP_598415
UniProt Q64314
研究領(lǐng)域 Hematopoietic Progenitors, Hematopoietic Stem Cells, Cardiogenesis, Lineage Markers, CD Antigens, Surface Receptors of Immune Cells

CD34 Molecule (CD34) 抗體 (PE)使用細節(jié)

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應(yīng)用備注 Flow Cytometry: Use 10 μL of Neat-1/5 diluted antibody to label 10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. 
Other applications not tested. 
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 For Research Use only

CD34 Molecule (CD34) 抗體 (PE)貯存及處理

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溶解方式 Restore with 0.25?mL (SM1603RT) or 1.0?mL (SM1603R) distilled water.
緩沖液 PBS,?pH 7.4 containing 0.09?% Sodium Azide as preservative, 5?% Sucrose and 1?% BSA as stabilizer.
儲存液 Sodium azide
注意事項 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事項 This product is photosensitive and should be protected from light
儲存條件 4 °C
儲存方法 Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!

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