Transferrin （Tf） 抗體 返回列表頁

Transferrin (Tf) 抗體

抗原
Transferrin (Tf)
別名
ltf, pro1557, pro2086, mgc107777, LOC692564, LOC100144362, tf, tf-b, MGC64306, cb285, gavi, id:ibd3238, id:ibd3525, sb:cb285, wu:fb57g06, wu:fb62h02, wu:fb63h10, wu:fb64h10, zgc:112154, 143958_at, CG6 ... show more
ltf, pro1557, pro2086, mgc107777, LOC692564, LOC100144362, tf, tf-b, MGC64306, cb285, gavi, id:ibd3238, id:ibd3525, sb:cb285, wu:fb57g06, wu:fb62h02, wu:fb63h10, wu:fb64h10, zgc:112154, 143958_at, CG6186, DmelCG6186, TSF1, Tf, Trf, anon-EST:Posey265, tsf1, TF, PRO1557, PRO2086, TFQTL1, AI266983, Cd176, HP, Tfn, hpx, LTF, TFEW, conalbumin show less
適用
其他選擇 Human
Human(301),Pig (Porcine) (39),Rat ((Rattus)) (38),Murine (28),Bovine (12),Rabbit (8),Duck (6),Sheep (Ovine) (5),Wild boar (Sus scrofa) (5),Horse (Equine) (3),Tag (3),Chinese Hamster (2),Cat (1),Guinea Pig (1),Hamster (1),Monkey (1)
宿主
其他選擇 Sheep
Rabbit(210),Mouse (122),Goat (50),Sheep (21),Chicken (18),Duck (2)
克隆類型
多克隆
標記
其他選擇 HRP
Biotin(39),HRP (23),FITC (21),Alexa Fluor 350 (2),Alexa Fluor 488 (2),Alexa Fluor 555 (2),Alexa Fluor 647 (2),Cy3 (2),Cy5 (2),Cy5.5 (2),Cy7 (2),Texas Red (TR) (2)
應(yīng)用范圍
其他選擇 Enzyme Immunoassay (EIA)
ELISA(241),Western Blotting (WB) (205),Immunohistochemistry (IHC) (74),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (67),Immunocytochemistry (ICC) (53),Immunoprecipitation (IP) (46),Immunofluorescence (IF) (39),Immunohistochemistry (Frozen Sections) (IHC (fro)) (38),Immunodiffusion (ID) (33),Enzyme Immunoassay (EIA) (29),Immunoelectrophoresis (IEP) (22),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (20),Radioimmunoassay (RIA) (17),Functional Studies (Func) (15),Magnetic Beads (MB) (11),Nephelometry (Neph) (9),Radial Immunodiffusion (RID) (9),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (8),Dot Blot (DB) (5),Flow Cytometry (FACS) (5),Immunoassay (IA) (4),Protein Assay (PrA) (3),ELISA (Detection) (2),ELISA (Capture) (1),Fluorescence Microscopy (FM) (1),Gel Diffusion (GD) (1),Immunomicroscopy (IM) (1),Immunoturbidimetry (IT) (1),Labeling (Lbl) (1),Rocket IEP (RIE) (1),Thin Layer Immunoassay (TIA) (1)
規(guī)格 1 mL
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Transferrin (Tf) 抗體

產(chǎn)品細節(jié)

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產(chǎn)品細節(jié)

顯示 隱藏
免疫原 The immunogen used was Human Holo-Transferrin purified from human serum and shown to be homogeneousby SDS-PAGE.
亞型 IgG
特異性 The unconjugated immunoglobulin gives a single arc when tested by immunoelectrophoresis and 2D-IEPagainst human serum.Identity has been confirmed by double diffusion (Ouchterlony) against human serum and an anti-humantransferrin of known specificity.
產(chǎn)品特性 Cellular Localization: Secreted
純化方法 IgG fraction. Antiserum is prepared by immunization of sheep with the above immunogen and if necessary, adsorption to monospecificity by the use of solid phase adsorbants. An immunoglobulin fraction is thenproduced by ion-exchange chromatography and zone electrophoresi.

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背景 Transferrin is a single polypeptide chain glycoprotein, and is a member of the iron binding family of proteins. It has a molecular weight of 79.5 kDa and a serum concentration range of 1800 - 2700 mg/L. It is synthesized in the liver and consists of two domains each having a high affinity reversible binding site forFe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in hemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions.
Synonyms: PRO1557, TF, PRO2086, 7018, P02787, Transferrin, 190000
研究領(lǐng)域 Serum/Plasma Proteins, Enzymes, Metabolism

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Transferrin (Tf) 抗體使用細節(jié)

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應(yīng)用備注 For enzyme immunoassays, to give an A450nm = 1.0 using wells coated with transferrin at 2ug/mLand TMB as a substrate: 1/12800-1/25600The titres reported should be used as guidelines for determining the working dilution in the userssystem.The following points should be observed when using this product:1) Dilutions of the conjugate must be made in a suitable buffer (e.g. 0.15M Phosphate buffered saline,pH7.2)2) It is recommended that dilutions of the conjugate are made in polypropylene or polyethylene containers.Other materials (e.g. polystyrene, some grades of glass) may be detrimental to the conjugate.3) Working dilutions of the conjugate should be prepared immediay prior to use.4) Peroxidase conjugates are adversely affected by the presence of sodium azide, the use of which shouldtherefore be avoided.5) The addition of Tween 20 (0.05-0.1%) to the conjugate diluent buffer is recommended to prevent nonspecificbinding in EIA. It may also be advantageous in other assay systems.
限制 For Research Use only

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狀態(tài) Liquid
濃度 5 mg/mL
緩沖液 PBS pH7.2,ProclinTM300 (0.05%)
儲存液 ProClin
儲存條件 4 °C
儲存方法 Keep as concentrated solution, aliquot and store at 4°C.

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