Collagen, Type IV （COL4） 抗體 返回列表頁

Collagen, Type IV (COL4) 抗體

抗原
Collagen, Type IV (COL4)
別名
1209, 6072, CG16858, CT25584, Coll IValpha2, DmColA2, DmelCG16858, Vkg, VkgC, alpha(IV)2/vkg, col4a2, coll. IV, l(2)01209, CG25C, CG4145, CT12803, Cgc25, Cgc25C, Coll, Coll IV, DCg1, Dcg, Dcg1, Dmel ... show more
1209, 6072, CG16858, CT25584, Coll IValpha2, DmColA2, DmelCG16858, Vkg, VkgC, alpha(IV)2/vkg, col4a2, coll. IV, l(2)01209, CG25C, CG4145, CT12803, Cgc25, Cgc25C, Coll, Coll IV, DCg1, Dcg, Dcg1, DmelCG4145, alpha(IV)1/Cg25C, alpha1, cg25C, cg25c, col4a1, gp125 show less
適用
其他選擇 Pig (Porcine)
Human(176),Murine (52),Bovine (45),Rat ((Rattus)) (34),Pig (Porcine) (33),Dog (Canine) (25),Chicken (12),Mammalian (4),Primate (2),Cat (1),Fish (1),Orang-Utan (1)
宿主
其他選擇 Rabbit
Rabbit(110),Mouse (79),Goat (16),Bovine (1)
克隆類型
多克隆
標記
其他選擇 非結合性
Biotin(16),FITC (4),Alexa Fluor 350 (2),Alexa Fluor 488 (2),Alexa Fluor 555 (2),Alexa Fluor 647 (2),Cy3 (2),Cy5 (2),Cy5.5 (2),Cy7 (2),HRP (2),PE (2)
應用范圍
其他選擇 Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Radioimmunoassay (RIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))(105),ELISA (104),Immunohistochemistry (Frozen Sections) (IHC (fro)) (91),Western Blotting (WB) (75),Immunofluorescence (IF) (49),Immunohistochemistry (IHC) (32),Radioimmunoassay (RIA) (25),Enzyme Immunoassay (EIA) (22),Immunocytochemistry (ICC) (21),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (20),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (18),Immunoprecipitation (IP) (16),Dot Blot (DB) (13),Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3),Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) (2),Immunomicroscopy (IM) (2),Flow Cytometry (FACS) (1),Immunoassay (IA) (1),Solid Phase Radioimmunoassay (SP-RIA) (1)
規(guī)格 0.5 mg
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發(fā)貨時間 29至35個工作日

Collagen, Type IV (COL4) 抗體

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產品細節(jié)

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免疫原 Purified collagen type IV from porcine renals.
特異性 This antibody is specific for Collagen type IV (100?% ). Porcine Collagen type I, II, III, Elastin, Fibronectin and Laminin < 0.1?% (in RIA at 1/1000 dilution).
純化方法 Affinity Chromatography.

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Collagen, Type IV (COL4) 抗體目標詳細情況

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別名 Collagen Type IV
背景 Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type IV Collagen (170-180 kDa), is a non-fibrilary network of different alpha-chains. It is typically found in basal membranes of different organs (e.g. skin, lens, lung, renals).Synonyms: COL4A1
基因ID 1282
NCBI Accession NP_001836
UniProt P02462
研究領域 Extracellular Matrix

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Collagen, Type IV (COL4) 抗體使用細節(jié)

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應用備注 ELISA: > 1/200 gives OD > 500. RIA. Immunofluorescence (1/80). Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/500. Incubation time: 60?min. at RT or 2-8?°C overnight. Positive Control: Porcine renals. Pretreatment: After de-waxing the tissue slices they are treated with 0.2?% hyaluronidase(app.300 U/mg) in TBS 15?min at 37?°C. There after non-specific binding is blocked byblocking serum or 3?% BSA in TBS. For peroxidase systems blocking with 1?% peroxidesolution in TBS for 30?min at RT is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 For Research Use only

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溶解方式 Restore with 0.5?mL distilled water. For further dilution use appropriate antibody diluent.
濃度 1.0 mg/mL
緩沖液 PBS without BSA and preservatives.
儲存液 Without preservative
注意事項 Avoid repeated freezing and thawing.
儲存條件 -20 °C

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