IL-6 抗體 返回列表頁

IL-6 抗體

抗原
IL-6
別名 Il-6, ILg6, Ifnb2, BSF2, HGF, HSF, IFNB2, IL-6, CHIL-6, il6
適用
其他選擇 Rat ((Rattus))
Human(355),Murine (146),Rat ((Rattus)) (88),Pig (Porcine) (28),Bovine (26),Dog (Canine) (24),Sheep (Ovine) (8),Rhesus Monkey (6),Goat (4),Horse (Equine) (4),Wild boar (Sus scrofa) (4),Chicken (3),Monkey (3),Rabbit (3),Baboon (2),Cynomolgus (2),Guinea Pig (1)
宿主
其他選擇 Rabbit
Rabbit(270),Mouse (173),Rat (101),Goat (17),Sheep (7),Guinea Pig (3),Chicken (1)
克隆類型
多克隆
標(biāo)記
其他選擇 非結(jié)合性
Biotin(67),FITC (26),HRP (14),PE (11),Alexa Fluor 350 (7),Alexa Fluor 488 (7),Alexa Fluor 555 (7),Alexa Fluor 647 (7),Cy3 (7),Cy5 (7),Cy5.5 (7),Cy7 (7),His tag (4),APC (3),Biotin,His tag,T7 tag (1),DyLight 488 (1),DyLight 550 (1),DyLight 594 (1),DyLight 650 (1),DyLight 755 (1),Magnetic Particles (1),PerCP (1),Sepharose (1)
應(yīng)用范圍
其他選擇 Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
Western Blotting (WB)(353),ELISA (298),Immunohistochemistry (IHC) (80),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (75),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (68),Neutralization (Neut) (67),Flow Cytometry (FACS) (66),Immunocytochemistry (ICC) (43),Enzyme Immunoassay (EIA) (40),Immunoprecipitation (IP) (40),Immunohistochemistry (Frozen Sections) (IHC (fro)) (36),Functional Studies (Func) (30),Immunofluorescence (IF) (28),Immunoassay (IA) (18),ELISpot (16),Blocking Antibody (Inhibition) (15),Radioimmunoassay (RIA) (13),Dot Blot (DB) (12),Cellular Assay (CA) (10),Immunostaining (IS) (10),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (6),ELISA (Detection) (3),ELISA (Capture) (2),ELISpot (Detection) (2),Intracellular Staining (ICS) (2),Transfected cell culture (TCC) (2),ELISpot (Capture) (1),In vivo Cytokine Capture Assay (IVCCA) (1),Inhibition Assay (InhA) (1),Proximity Ligation Assay (PLA) (1)
[Pubmed] 3 references available
規(guī)格 0.1 mg
發(fā)貨至 中國 (更改)
發(fā)貨時(shí)間 29至35個(gè)工作日

IL-6 抗體

產(chǎn)品細(xì)節(jié)

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產(chǎn)品細(xì)節(jié)

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免疫原 Highly pure (>98%) recombinant Rat IL-6
特異性 The antibody recognizes IL-6.
純化方法 Affinity chromatography employing immobilized Rat IL-6 matrix

目標(biāo)詳細(xì)情況

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目標(biāo)詳細(xì)情況

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別名 Interleukin-6 (IL6)
背景 Interleukin 6 (IL6) is a multifunctional 24 kD protein originally discovered in the medium of RNA stimulated fibroblastoid cells. It is upregulated by IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, IL17 and downregulated by glucocorticoids IL4, TGF beta. IL6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important as IL1 and TNF alpha in regulating the acute phase response. IL6 has also been implicated in regulating adipose mass. IL6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL6 appears to interact with IL2 in the proliferation of T lymphocytes. IL6 potentiates the proliferative effect of IL3 on multipotential hematopoietic progenitors.Synonyms: B-cell stimulatory factor 2, BSF-2, CDF, CTL differentiation factor, Hybridoma growth factor, IFNB2, IL-6, Interferon beta-2
基因ID 24498
NCBI Accession NP_036721
UniProt P20607
研究領(lǐng)域 Cytokines, Receptors, Inflammation, Immunology, Virology, Cancer

使用細(xì)節(jié)

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使用細(xì)節(jié)

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應(yīng)用備注 NeutralizationTo yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-6 (0.5?ng/mL),aconcentration of 1.0-1.5?μg/mL of this antibody is required. ELISAIndirect: To detect Rat IL-6 by indirect ELISA (using 100 ml/well antibody solution) aconcentration of 0.5-2.0?mg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4?ng/well of recombinant Rat IL-6. Sandwich: To detect Rat IL-6 by sandwich ELISA (using 100 ml/well antibody solution) aconcentration of 0.5-2.0?mg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with a biotinylated Anti-Rat IL-6 (PP036B1 or PP036B1) as adetection antibody, allows the detection of at least 0.2-0.4?ng/well of recombinant RatIL-6.
限制 For Research Use only

貯存及處理

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貯存及處理

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溶解方式 Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0?mg/mL.
緩沖液 PBS,?pH 7.2
注意事項(xiàng) Avoid repeated freezing and thawing.
儲(chǔ)存條件 4 °C/-20 °C
儲(chǔ)存方法 The lyophilized antibody is stable for one year from date of despatch at -20 °C. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
有效期 12 months

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