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SPOP抗體

抗原
Speckle-Type POZ Protein (SPOP)
別名 tef2, SPOP, spop, zgc:64140, BTBD32, TEF2, AI315626, Pcif1
抗原表位
其他選擇 C-Term
AA 85-115(7),N-Term (6),AA 1-374 (2),C-Term (2),AA 1-223 (1),AA 139-354 (1),AA 301-374 (1)
適用
其他選擇 Murine, Rat ((Rattus)), Bovine, Xenopus laevis, Dog (Canine), Zebrafish (Danio rerio), Human, Chicken
Human(30),Murine (13),Rat ((Rattus)) (13),Chicken (1),Bovine (1),Dog (Canine) (1),Xenopus laevis (1),Zebrafish (1)
宿主
其他選擇 Rabbit
Rabbit(25),Mouse (3),Chicken (2)
克隆類型
多克隆
標(biāo)記
其他選擇 非結(jié)合性
Biotin(2),FITC (2),HRP (2),APC (1),Alexa Fluor 350 (1),Alexa Fluor 488 (1),Alexa Fluor 555 (1),Alexa Fluor 647 (1),Alkaline Phosphatase (AP) (1),Cy3 (1),Cy5 (1),Cy5.5 (1),Cy7 (1),PE (1)
應(yīng)用范圍
其他選擇 Western Blotting (WB)
Western Blotting (WB)(21),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (10),ELISA (8),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3),Immunoassay (IA) (2),Immunofluorescence (IF) (1),Immunohistochemistry (IHC) (1)
[Pubmed] 1 reference available
規(guī)格 50 μg
發(fā)貨至 中國 (更改)
發(fā)貨時(shí)間 9至12個(gè)工作日

SPOP抗體

產(chǎn)品細(xì)節(jié)

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產(chǎn)品細(xì)節(jié)

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免疫原 Synthetic peptide directed towards the C terminal of human SPOP
序列 YHASDVLETS GWKSMVVSHP HLVAEAYRSL ASAQCPFLGP PRKRLKQS
預(yù)測反應(yīng) African clawed frog : 100 %, Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, Zebrafish : 100 %
產(chǎn)品特性 This is a rabbit polyclonal antibody against SPOP. It was validated on Western Blot using a cell lysate as a positive control.
純化方法 Affinity Purified

目標(biāo)詳細(xì)情況

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目標(biāo)詳細(xì)情況

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別名 SPOP
背景 SPOP may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein.
分子量 42 kDa
基因ID 8405
NCBI Accession NM_001007226, NP_001007227
UniProt O43791
研究領(lǐng)域 Chromatin and Nuclear Signaling, DNA/RNA

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使用細(xì)節(jié)

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應(yīng)用備注 Optimal working dilutions should be determined experimentally by the investigator.
說明

Antigen size: 374 AA

限制 For Research Use only

貯存及處理

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貯存及處理

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狀態(tài) Lyophilized
溶解方式 Add 50 µL of distilled water.
濃度 1 mg/mL
緩沖液 PBS buffer with 2 % sucrose
注意事項(xiàng) Avoid repeated freeze-thaw cycles.
儲(chǔ)存條件 -20 °C
儲(chǔ)存方法 For longer periods of storage, store at -20 °C

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