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CST7 抗體

抗原
Cystatin F (Leukocystatin) (CST7)
別名 CST7, LOC100232568, cytf, CMAP, Cmap
抗原表位
其他選擇 AA 1-145
AA 1-145(8),C-Term (5),N-Term (1)
適用
其他選擇 Human
Human(29),Murine (16),Rat ((Rattus)) (15),Dog (Canine) (1),Horse (Equine) (1),Pig (Porcine) (1),Rabbit (1)
宿主
其他選擇 Mouse
Rabbit(21),Goat (7),Mouse (3)
克隆類(lèi)型 (克隆位點(diǎn))
單克隆 (2F11D9B2)
標(biāo)記
其他選擇 非結(jié)合性
Alexa Fluor 350(1),Alexa Fluor 488 (1),Alexa Fluor 555 (1),Alexa Fluor 647 (1),Biotin (1),Cy3 (1),Cy5 (1),Cy5.5 (1),Cy7 (1),FITC (1),HRP (1)
應(yīng)用范圍
其他選擇 Western Blotting (WB)
Western Blotting (WB)(16),ELISA (11),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (10),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5),Immunohistochemistry (IHC) (4)
規(guī)格 50 μg
發(fā)貨至 中國(guó) (更改)

CST7 抗體

產(chǎn)品細(xì)節(jié)

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產(chǎn)品細(xì)節(jié)

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免疫原 Recombinant human CST7 protein
克隆位點(diǎn) 2F11D9B2
亞型 IgG1
特異性 Human Cystatin-F / CST7 / Cystatin-7
交叉反應(yīng) （詳細(xì)） No cross-reactivity with human cell lysate (293 cell line) in WB and ELISA
產(chǎn)品特性 This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human CST7 ( rh CST7 , NP_003641.2 , Met 1 - His 145 ). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
純化方法 Affinity Purified
過(guò)濾 0.2 μm filtered

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目標(biāo)詳細(xì)情況

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別名 CST7
背景 The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis.
NCBI Accession NP_003641.2

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應(yīng)用備注 Western blot - WB: 2-10 μg/mL
限制 For Research Use only

貯存及處理

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貯存及處理

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緩沖液 PBS with 5% trehalose
儲(chǔ)存液 Without preservative
注意事項(xiàng) Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
儲(chǔ)存條件 4 °C/-20 °C/-80 °C
儲(chǔ)存方法 This antibody can be stored at 2 °C-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C.
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