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CD4 抗體

抗原
CD4 Molecule (CD4)
別名 cd4, L3T4, Ly-4, CD4mut, W3/25, p55
抗原表位
其他選擇 AA 1-390
N-Term(50),C-Term (18),Internal Region (15),AA 1-401 (10),Domain 1 (10),AA 1-390 (9),Transcript Variant 1 (9),pSer433 (7),AA 336-366 (6),AA 57-83 (6),AA 63-93 (6),Extracellular (6),Domain 2 (5),Non-Polymorphic Site (5),AA 1-394 (4),AA 26-226 (3),AA 26-390 (3),AA 27-394 (3),Domain 3 (3),Domain 4 (3),Domain D1 (3),AA 26-396 (2),AA 408-436 (2),AA 414-433 (2),AA 58-86 (2),Extracellular Domain (2),Middle Region (2),AA 1-221 (1),AA 1-458 (1),AA 172-414 (1),AA 28-394 (1),AA 325-375 (1),Ala429 (1),Cytoplasmic Domain (1),Ser213 (1),Ser433 (1),Ser706 (1),full length (1)
適用
其他選擇 Human
Human(683),Murine (549),Rat ((Rattus)) (162),Chicken (43),Dog (Canine) (33),Cat (28),Bovine (25),Monkey (24),Guinea Pig (23),Pig (Porcine) (20),Sheep (Ovine) (17),Ferret (10),Rabbit (8),Mammalian (5),Rhesus Monkey (4),Duck (3),Goat (3),Horse (Equine) (3),Zebrafish (1)
宿主
其他選擇 Rabbit
Mouse(779),Rat (487),Rabbit (179),Goat (3),Human (1),Sheep (1)
克隆類型
多克隆
標(biāo)記
其他選擇 非結(jié)合性
FITC(213),PE (185),Biotin (149),APC (77),Cy5 (18),PerCP (15),PE-Cy5 (13),HRP (10),PE-Cy7 (10),PerCP,Cy5.5 (10),Allophycocyanin (9),Cy5,PE (9),SPRD (7),Alexa Fluor 700 (6),Cy7,PE (6),PE,Cy5 (6),PE-Cy5.5 (6),APC-Cy7 (5),Alexa Fluor 488 (5),Alkaline Phosphatase (AP) (4),PE,Cy7 (4),APC-Cy5.5 (3),Alexa Fluor 350 (3),Alexa Fluor 555 (3),Alexa Fluor 647 (3),Cy3 (3),Cy5.5 (3),Cy7 (3),DY647 (3),DyLight 680 (3),Magnetic Particles (3),APC eFluor 780 (2),DyLight 488 (2),DyLight 550 (2),DyLight 594 (2),DyLight 650 (2),EFluor 450 (2),PE-DyLight 594 (2),PE-TR (2),Texas Red (TR) (2),CF405M (1),DyLight 755 (1),PE,DY590 (1),PE,DY647 (1),Pacific Blue (1),PerCP-Cy5.5 (1),Quantum Red (1),Sepharose (1)
zui低檢測(cè)濃度 0.0195 ng/well
應(yīng)用范圍
其他選擇 ELISA
Flow Cytometry (FACS)(1102),Western Blotting (WB) (248),Immunohistochemistry (IHC) (235),ELISA (168),Immunohistochemistry (Frozen Sections) (IHC (fro)) (165),Immunoprecipitation (IP) (158),Immunofluorescence (IF) (101),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (101),Immunocytochemistry (ICC) (57),Functional Studies (Func) (50),Immunohistochemistry (Acetone-fixed) (IHC (af)) (32),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (30),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (21),Cytotoxicity Test (CyTox) (12),Enzyme Immunoassay (EIA) (7),Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (7),Blocking Reagent (BR) (5),Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3),Neutralization (Neut) (3),Immunoassay (IA) (2),Immunohistochemistry (Fixed) (IHC (fx)) (2),Immunostaining (IS) (2),Activation (Act) (1),Blocking Antibody (Inhibition) (1),Fluorescence Microscopy (FM) (1),Immunoelectron Microscopy (IEM) (1),Indirect Immunofluorescence Assay (IFA) (1)
[Pubmed] 1 reference available
規(guī)格 100 μg
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CD4 抗體

產(chǎn)品細(xì)節(jié)

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產(chǎn)品細(xì)節(jié)

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亞型 IgG
特異性 Human CD4 / Leu-3
產(chǎn)品特性 Produced in rabbits immunized with purified, recombinant Human CD4 / Leu-3 (rh CD4 / Leu-3, NP_000607.1, Met 1-Trp 390). Total IgG was purified by Protein A affinity chromatography.
純化方法 Affinity Purified
過(guò)濾 0.2 μm filtered

目標(biāo)詳細(xì)情況

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目標(biāo)詳細(xì)情況

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別名 CD4
背景 T-cell surface glycoprotein CD4, is a single-pass type I membrane protein. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. The CD4 surface determinant, previously associated as a phenotypic marker for helper/inducer subsets of T lymphocytes, has now been critically identified as the binding/entry protein for human immunodeficiency viruses (HIV). The human CD4 molecule is readily detectable on monocytes, T lymphocytes, and brain tissues. All human tissue sources of CD4 bind radiolabeled gp120 to the same relative degree, however, the murine homologous protein, L3T4, does not bind the HIV envelope protein. CD4 is a co-receptor that assists the T cell receptor (TCR) to activate its T cell following an interaction with an antigen presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR. CD4 interacts directly with MHC class II molecules on the surface of the antigen presenting cell via its extracellular domain. The CD4 molecule is currently the object of intense interest and investigation both because of its role in normal T-cell function, and because of its role in HIV infection. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Viral protein U (VpU) of HIV-1 plays an important role in downregulation of the main HIV-1 receptor CD4 from the surface of infected cells. Physical binding of VpU to newly synthesized CD4 in the endoplasmic reticulum is an early step in a pathway leading to proteasomal degradation of CD4. Amino acids in both helices found in the cytoplasmic region of VpU in membrane-mimicking detergent micelles experience chemical shift perturbations upon binding to CD4, whereas amino acids between the two helices and at the C-terminus of VpU show no or only small changes, respectively. Paramagnetic spin labels were attached at three sequence positions of a CD4 peptide comprising the transmembrane and cytosolic domains of the receptor. VpU binds to a membrane-proximal region in the cytoplasmic domain of CD4.
NCBI Accession NP_000607.1
研究領(lǐng)域 Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells

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使用細(xì)節(jié)

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應(yīng)用備注 ELISA ELISA: 0.5-1 μg/mL This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CD4. The detection limit for Human CD4 is approximay 0.0195 ng/well.
限制 For Research Use only

貯存及處理

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貯存及處理

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緩沖液 PBS with 5% trehalose
儲(chǔ)存液 Without preservative
注意事項(xiàng) Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
儲(chǔ)存條件 4 °C/-20 °C/-80 °C
儲(chǔ)存方法 This antibody can be stored at 2 °C-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C.
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