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TGFB1抗體

抗原
Transforming Growth Factor, beta 1 (TGFB1)
別名
TGF-beta, ai39657, tgfb1, wu:fb13a07, xx:ai39657, TGFB, TGFbeta, TGFB1, TGFBI, csd, cdb1, cdg2, csd1, csd2, csd3, ebmd, lcd1, bigh3, cdgg1, betaig-h3, MGC146225, TGFB4, CED, DPD1, LAP, TGF-BETA-1, Tgf ... show more
TGF-beta, ai39657, tgfb1, wu:fb13a07, xx:ai39657, TGFB, TGFbeta, TGFB1, TGFBI, csd, cdb1, cdg2, csd1, csd2, csd3, ebmd, lcd1, bigh3, cdgg1, betaig-h3, MGC146225, TGFB4, CED, DPD1, LAP, TGF-BETA-1, Tgfb, TGF-beta1, TGFbeta1, Tgfb-1, TGF-beta5, ced, dpd1, lap, tgf-beta, tgfb, tgfb5, tgfbeta, tgf show less
抗原表位
其他選擇 AA 279-390
AA 279-390(27),N-Term (9),AA 177-391 (7),AA 286-293 (6),AA 29-58 (5),C-Term (5),Middle Region (4),AA 30-278 (3),AA 30-390 (3),Internal Region (3),AA 1-111 (2),AA 22-50 (2),AA 62-195 (2),AA 1-269 (1),AA 1-390 (1),AA 1-99 (1),AA 260-373 (1),AA 280-391 (1),AA 336-385 (1),AA 8-15 (1),Mature (1),Tyr369 (1)
適用
其他選擇 Human
Human(188),Murine (50),Rat ((Rattus)) (45),Pig (Porcine) (27),Bovine (17),Dog (Canine) (17),Guinea Pig (16),Xenopus laevis (12),Chicken (4),Rabbit (4),Wild boar (Sus scrofa) (4),Zebrafish (4),Mammalian (2),Sheep (Ovine) (2),Amphibian (1),Goat (1),Horse (Equine) (1)
宿主
其他選擇 Mouse
Rabbit(167),Mouse (71),Goat (4),Chicken (3),Rat (2),Guinea Pig (1),Sheep (1)
克隆類型 (克隆位點)
單克隆 (1G9H11G3)
標記
其他選擇 非結合性
Biotin(17),FITC (12),HRP (7),APC (4),PE (4),Alexa Fluor 350 (3),Alexa Fluor 488 (3),Alexa Fluor 555 (3),Alexa Fluor 647 (3),Cy3 (3),Cy5 (3),Cy5.5 (3),Cy7 (3),His tag,T7 tag (2),Alkaline Phosphatase (AP) (1),CF (1)
應用范圍
其他選擇 Western Blotting (WB)
Western Blotting (WB)(171),ELISA (130),Immunohistochemistry (IHC) (79),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (58),Immunocytochemistry (ICC) (33),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (30),Immunohistochemistry (Frozen Sections) (IHC (fro)) (28),Immunofluorescence (IF) (21),Flow Cytometry (FACS) (19),Enzyme Immunoassay (EIA) (12),Neutralization (Neut) (12),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (10),Functional Studies (Func) (4),Proximity Ligation Assay (PLA) (2),Blocking Antibody (Inhibition) (1),Fluorescence Microscopy (FM) (1),Immunoassay (IA) (1),Immunoprecipitation (IP) (1),Inhibition Assay (InhA) (1)
規(guī)格 50 μg
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TGFB1抗體

產品細節(jié)

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產品細節(jié)

顯示 隱藏
免疫原 Recombinant human TGF-beta1 protein
克隆位點 1G9H11G3
亞型 IgG1
特異性 Human TGF-beta1 / TGFB1.
交叉反應 （詳細） No cross-reactivity with human cell lysate (293 cell line) in WB and ELISA.
產品特性 This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human TGF-beta1 / TGFB1 ( rh TGF-beta1 , Ala 279 - Ser 390 , NP_000651.3 ). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
純化方法 Affinity Purified
過濾 0.2 μm filtered

目標詳細情況

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目標詳細情況

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別名 TGFB1
背景 Transforming growth factor beta 1 (TGF-beta1, TGFβ1) is a member of the large transforming growth factor beta superfamily of cytokines, and serves as a multifunctional molecule performing actions in many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. TGFβ1 was first synthesized as a 390 amino acid precusor. The secreted proprotein contains 360 aa, and is cleaved into mature TGFβ1 (112 aa) and latency-associated peptide (LAP)(248 aa), which remains non- covalently linked to mature TGFβ1 rendering it inactive. TGFβ1 is synthesized by many cells, and regulates many other growth factors both in positive and negative manner. It has been shown that TGFβ1 plays an important role in controlling the immune system and shows different activities on different types of cell, or cells at different developmental stages. It is highly expressed in bone and as a potent stimulator of osteoblastic bone formation. TGFβ1 is able to cause chemotaxis, proliferation and differentiation in committed osteoblasts. TGFβ1 is also identified as a disease marker and a therapeutical target for cardiovascular disease
NCBI Accession NP_000651.3

使用細節(jié)

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使用細節(jié)

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應用備注 Western blot - WB: 5-20 μg/mL
限制 For Research Use only

貯存及處理

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貯存及處理

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緩沖液 PBS with 5% trehalose
儲存液 Without preservative
注意事項 Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
儲存條件 4 °C/-20 °C/-80 °C
儲存方法 This antibody can be stored at 2 °C-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C.
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