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The Acclaim® Mixed-Mode HILIC-1 column utilizes a unique silica-based, mixed-mode stationary phase that combines both reversed-phase (RP) and hydrophilic interaction liquid chromatography (HILIC) properties. The column functional group features a hydrophobic alkyl chain and hydrophilic diol functionality. This combination allows both hydrophobic interaction and hydrophilic interaction to be utilized to optimize separations.
Operates in both RP and HILIC modes
Retains highly polar molecules
Unique selectivity complementary to RP columns
Superior performance compared to conventional diol-based columns
High efficiency column for high resolution separations
The Acclaim Mixed-Mode HILIC-1 columns consist of 5 μm high-purity, porous, spherical silica with 120 Å-diameter pores bonded with proprietry functional groups, which provide unsurpassed resolution and peak symmetry for a variety of polar and nonpolar molecules.
Wide Range of Applications
The Acclaim Mixed-Mode HILIC-1 column separates both polar and nonpolar molecules with selectivity complementary to RP columns and is suitable for a broad range of applications, including drug metabolites, lipids, polyethylene glycols (PEGs), ethoxylated surfactants and more.
| Acclaim Mixed-Mode HILIC-1 Columns Specifications | |
|---|---|
| Particle size: | 5 µm |
| Base silica: | Spherical, high-purity |
| Pore size: | 120 Å |
| Surface area: | 300 m2/g |
| Column chemistry: | Proprietary alkyl diol |