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廣州尖分科學(xué)儀器有限公司>>戴安 Dionex>>Bio Columns>> MAbPac SEC-1 Monoclonal Antibody Analysis Column

MAbPac SEC-1 Monoclonal Antibody Analysis Column

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更新時(shí)間:2016-10-27 15:56:26瀏覽次數(shù):256

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MAbPac SEC-1 Monoclonal Antibody Analysis ColumnMAbPac™ SEC-1 is a size exclusion chromatography (SEC) column designed for monoclonal antibody (MAb) analysis, including monomers,

詳細(xì)介紹

MAbPac SEC– 1 for Monoclonal Antibody (MAb) Analysis

MAbPac SEC-1 is a size exclusion chromatography (SEC) column designed for monoclonal antibody (MAb) analysis, including monomers, aggregates, and fragments, under non-denaturing conditions, and in both high- and low-salt phases and volatile eluents.

• Proprietary hydrophilic bonded layer results in minimal non-desired interactions between the biomolecules and the stationary phase.
• Nonmetallic and Biocompatible PEEK housing eliminates metal contamination from the column hardware.
• Stable surface bonding leads to low column bleed and compatibility with MS, ELSD and Corona® CAD detection.
• Rugged, reproducible column.
• Superior performance for the analysis of monoclonal antibodies, even at low salt concentrations.


Applications
SEC is frequently used because of its simple operating principle and features not found in other separation techniques including separating aggregates that are not easily distinguished by other chromatographic means. MAbs produced from mammalian cell culture may contain significant amounts of dimers and higher-order aggregates. The MAbPac SEC-10 is a high-resolution SEC column that is suitable for use with a wide range of biomolecules, including monoclonal antibodies and their aggregates.

The MAbPac SEC-1 column has a wide molecular operating range as shown in the molecular weight calibration curve. 

ProteinMAbPac SEC-1
Retention Time (min)
MW (kDa)
γ-Globulin aggregate7.67,000
Thyroglobulin dimer8.01,338
Thyroglobulin8.9669
γ-Globulin dimer10.3300
BSA dimer11.4134
BSA12.667
Ovalubumin13.343
Trypsin Inhibitor14.122
Myoglobin14.418
Ribonuclease A14.714
Cytochrome C14.612

 

The MAbPac SEC-1 column can be used for quantitative separation of a monoclonal antibody and its aggregates.

Faster MAb size-exclusion separation can be achieved using a shorter column.

 

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