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lycanPac AXR-1 Columns for the High-Resolution Separation and Analysis of Glycans
The Thermo Scientific™ GlycanPac™ AXR-1 column is a high-performance, silica-based HPLC column for the simultaneous separation of glycans by charge, isomerism, and size. It is designed to provide industry-leading resolution with unique selectivities for biologically important glycans, either labeled or native, using either fluorescence and/or mass spectrometry (MS) detection.
Features:
• Unique glycan selectivity based on charge, branch/linkage isomerism, and size
• Exceptional resolution for either native or derivatized glycans
• Utility for both high-resolution glycan structural characterization and glycan quantification
• Compatibility with both MS and fluorescence detection methods
• Reliable column performance
• Available in HPLC (3 µm) and UHPLC (1.9 µm) formats.
The GlycanPac AXR-1 column, based on innovative, mixed-mode surface chemistry, combines both weak anion-exchange (WAX) and reversed-phase interaction mechanisms to produce exceptional glycan selectivity and industry-leading resolution. The WAX functionality provides retention of negatively charged glycans that elute in order of increasing charge, and the reversed-phase mode facilitates resolution of glycans of the same charge according to their isomerism and size. These attributes produce unparalleled glycan separations. The GlycanPac AXR-1 column is designed for HPLC and UHPLC methods using either fluorescence or MS detection, and uses volatile aqueous buffers (e.g., ammonium acetate or ammonium formate) and acetonitrile, presenting the eluting glycans ready for introduction into MS instruments.
GlycanPac AXR-1 columns are based on high-purity and spherical silica substrates in both 1.9 and 3 µm sizes for UHPLC and standard HPLC applications, respectively. GlycanPac AXR-1 columns are available in various dimensions for applications with different resolutions, throughput, and instrument requirements.
| GlycanPac AXR-1 Column Specifications | |
|---|---|
| Column Dimensions | 1.9 μm: 2.1 × 250mm, 2.1 × 150mm 3 μm: 4.6 × 150mm, 3.0 × 150mm, 2.1 × 150mm |
| Particle Size | Spherical 1.9 μm, 3 μm particles |
| Substrate | High-purity, Porous, Spherical Silica |
| Surface Area | 220 m2/g |
| Pore size | 175 Å |
| Functional Group | WAX and RP Mixed-Mode |
| Recommended Flow Rate Range: | 1.9 μm: 0.2 to 0.4 mL/min. for: all 2.1 mm columns 3 μm: 0.8 to 1.2 ml/min for 4.6 × 150 mm 0.3 to 0.6 ml/min for 3.0 × 150 mm 0.1 to 0.3 ml/min. for 2.1 × 150 mm |
| Pressure Limit | 1.9 μm: 10,000 psi for 2.1 ×150mm 15,000 psi for 2.1 × 250mm 3 μm: 6,000 psi for all 3 μm columns |
| Temperature Range: | Ambient to < 60 ºC |
| pH Range | 2.0 - 8.0 |