轉(zhuǎn)化生長因子β1(TGFβ1)重組蛋白

時間:2016-1-11閱讀:624
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轉(zhuǎn)化生長因子β1(TGFβ1)重組蛋白

Recombinant Transforming Growth Factor Beta 1 (TGFb1)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism speciesBos taurus; Bovine (Cattle)
Product No.klA124Bo01
SourceProkaryotic expression
HostE.coli
Purity> 95%
UOM50ug
Predicted Molecular Mass45.2kDa
Concentration7.2
ApplicationsSDS-PAGE; WB; ELISA; IP.
Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
ResiduesAla279~Ser390 with two N-terminal Tags, His-tag and GST-tag

FormulationSupplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

轉(zhuǎn)化生長因子β1(TGFβ1)重組蛋白SEQUENCES

The sequence of the target protein is listed below.
AL DTNYCFSSTE KNCCVRQLYI DFRKDLGWKW IHEPKGYHAN FCLGPCPYIW SLDTQYSKVL ALYNQHNPGA SAAPCCVPQA LEPLPIVYYV GRKPKVEQLS NMIVRSCKCS

USAGE

Reconstitute in sterile PBS, pH7.2-pH7.4.

轉(zhuǎn)化生長因子β1(TGFβ1)重組蛋白STORAGE AND STABILITY

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

About the MARKER (complimentary)

轉(zhuǎn)化生長因子β1(TGFβ1)重組蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.

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