蛋白質(zhì)印跡（WB）用于測量不同條件下特定蛋白的變化。在蛋白質(zhì)印跡（WB）過程中涉及許多步驟，包括樣品制備、電泳、蛋白轉(zhuǎn)移、抗體孵育和信號(hào)檢測。為了解釋蛋白質(zhì)印跡實(shí)驗(yàn)的結(jié)果，一個(gè)貫穿全過程的內(nèi)參是必須的。
內(nèi)參可保證每個(gè)泳道中加入了相同量的蛋白樣品,不同泳道上的蛋白以相同的效率從凝膠轉(zhuǎn)到膜上,不同泳道上抗體孵育（對(duì)于一抗而言，必要的話也包括二抗）、信號(hào)檢測都是一致的。
內(nèi)參的信號(hào)通常用來對(duì)目標(biāo)蛋白的信號(hào)進(jìn)行標(biāo)準(zhǔn)化。需要強(qiáng)調(diào)的是，對(duì)照蛋白抗體應(yīng)該與實(shí)驗(yàn)抗體在相同的印跡上進(jìn)行。
不同的樣品制備需要不同的內(nèi)參。下面總結(jié)了針對(duì)不同樣品制備常用的內(nèi)參，包括全細(xì)胞/胞漿蛋白、線粒體、核蛋白、植物組織和血清樣本。
針對(duì)不同樣品制備常用的內(nèi)參以及它們的分子量
蛋白質(zhì)印跡（Western Blot）實(shí)驗(yàn)中產(chǎn)品推薦：
| 實(shí)驗(yàn)步驟 | 推薦指數(shù) | 貨號(hào) | 產(chǎn)品名稱 | 規(guī)格 |
| 樣本制備 | abs9228 | RIPA (中) | 100ml | |
| abs9229 | RIPA (強(qiáng)) | 100ml | ||
| abs9230 | RIPA (弱) | 100ml | ||
| abs9231 | RIPA (強(qiáng)，無抑制劑) | 100ml | ||
| abs9160 | NP-40 | 100ml | ||
| abs9195 | 1M Tris-HCl，pH6.8 | 1L | ||
| abs9201 | 1M Tris-HCl，pH8.8（更多pH請(qǐng)看愛必信網(wǎng)站） | 1L | ||
| abs9117 | SDS | 100ml | ||
| ★★ | abs9116 | WB/IP 細(xì)胞裂解液 | 100ml | |
| ★★ | abs9239 | WB/IP 細(xì)胞裂解液(無抑制劑) | 100ml | |
| ★★★★ | abs9101 | 紅細(xì)胞裂解液 | 100ml,250ml,500ml | |
| abs812852 | PMSF | 50mg,100mg,200mg | ||
| abs816442 | Aprotinin 抑肽酶 | 10mg,25mg,50mg,100mg | ||
| abs817902 | Peptatin 胃蛋白酶抑制劑A | 10mg,25mg,50mg,100mg | ||
| abs9133 | EDTA | 100ml | ||
| abs42017684 | EGTA | 1g,10g | ||
| abs817431 | 原釩酸鹽 | 100mg,500mg | ||
| abs47014865 | β-甘油磷酸鹽 | 10g,25g | ||
| ★★ | abs9161 | 廣譜型蛋白酶抑制劑混合物 | 1ml | |
| ★★ | abs9162 | 廣譜型磷酸酶抑制劑混合物 | 1ml | |
| 蛋白定量 | ★★ | abs9232 | BCA蛋白定量試劑盒 | 500次,2500次 |
| ★★ | abs9233 | 增強(qiáng)型BCA 蛋白定量試劑盒 | 500次 | |
| 上樣與電泳 | abs9226 | DTT溶液(1mol/L) | 5ml,10ml | |
| abs9237 | 2X Laemmli樣品緩沖液 | 5ml*2 | ||
| abs9238 | 10%SDS十二烷基硫酸鈉溶液 | 500ml | ||
| ★★ | abs953 | 2*上樣緩沖液 (適用于SDS-PAGE) | 10ml | |
| ★★ | abs9301 | 預(yù)制膠 10%,10 wells | 5片/盒 | |
| ★★ | abs9308 | 預(yù)制膠 10%,15 wells | 5片/盒 | |
| ★★ | abs9302 | 預(yù)制膠 12%,10 wells | 5片/盒 | |
| ★★ | abs9309 | 預(yù)制膠 12%,15 wells | 5片/盒 | |
| abs9303 | 預(yù)制膠 15% ,10 wells | 5片/盒 | ||
| abs9310 | 預(yù)制膠 15% ,15 wells | 5片/盒 | ||
| abs9304 | 預(yù)制膠 4-15%, 10 wells | 5片/盒 | ||
| abs9311 | 預(yù)制膠 4-15%, 15 wells | 5片/盒 | ||
| ★★ | abs9305 | 預(yù)制膠 4-20%, 10 wells | 5片/盒 | |
| ★★ | abs9312 | 預(yù)制膠 4-20%, 15 wells | 5片/盒 | |
| abs9307 | 預(yù)制膠 8%, 15 wells | 5片/盒 | ||
| abs9306 | 預(yù)制膠 8-20%, 10 wells | 5片/盒 | ||
| abs9313 | 預(yù)制膠 8-20%, 15 wells | 5片/盒 | ||
| NEW | abs9328 | 3-5kd小分子量預(yù)制膠，16.5%，10wells | 10片/盒 | |
| NEW | abs9329 | 3-5kd小分子量預(yù)制膠，16.5%，15wells | 10片/盒 | |
| ★★★ | abs925 | 蛋白電泳套裝 | 盒 | |
| abs951 | running buffer | 100ml,1L | ||
| ★★★★★ | abs923 | 預(yù)染蛋白marker, 10-245 KDa | 500ul,5*500ul | |
| ★★★★★ | abs924 | 預(yù)染蛋白marker, 10-180 kDa | 250ul,2*250ul | |
| abs966 | Genshare考馬斯亮藍(lán)蛋白膠快速染色液 | 500ml | ||
| ★★★ | abs964 | 考馬斯亮藍(lán)染色試劑盒(常規(guī)型)R250 | 1kit | |
| abs965 | 考馬斯亮藍(lán)染色試劑盒(增強(qiáng)型)G250 | 1kit | ||
| 轉(zhuǎn)膜 | abs931 | PVDF膜0.2 μm | 20張 | |
| ★★ | abs932 | PVDF膜0.45 μm | 20張 | |
| ★★ | abs959 | NC膜0.2 μm | 20張 | |
| abs960 | NC膜0.45 μm | 20張 | ||
| abs950 | 10*電轉(zhuǎn)液 | 100ml,1L | ||
| 封閉 | abs9174 | 脫脂奶粉 | 100g,500g | |
| ★★★★★ | abs9155 | BSA | 50g,100g | |
| 一抗孵育 | ★★ | abs830031 | Beta actin antibody | 50ul,100ul |
| abs132001 | Beta actin antibody | 50ug,100ug | ||
| abs119680 | Beta Actin Polyclonal Antibody | 50ul,100ul | ||
| abs132184 | Beta-Actin antibody | 50ug,100ug | ||
| abs137975 | Beta-Actin Mouse Monoclonal Antibody | 50ug,100ug | ||
| ★★ | abs830030 | GAPDH antibody | 50ul,100ul | |
| abs132004 | GAPDH antibody | 50ug,100ug | ||
| abs137959 | GAPDH Mouse Monoclonal Antibody | 50ug,100ug | ||
| abs118936 | GAPDH Polyclonal Antibody | 50ul,100ul | ||
| abs131993 | Tubulin alpha Antibody | 50ug,100ug | ||
| ★★ | abs830032 | Tubulin Beta antibody | 50ul,100ul | |
| abs131994 | Tubulin beta Antibody | 50ug,100ug | ||
| abs125209 | Tubulin Beta Polyclonal Antibody | 50ul,100ul | ||
| 二抗孵育 | ★★ | abs928 | Goat anti-mouse IgG-HRP | 100ul,500ul,1ml |
| ★★ | abs20002 | Goat anti-rabbit IgG-HRP | 100ul,500ul,1ml | |
| ★★ | abs20003 | Goat anti-Mouse IgG-FITC | 1ml,500ul,100ul | |
| ★★ | abs20004 | Goat anti-Rabbit IgG-FITC | 100ul,500ul,1ml | |
| ★★ | abs20005 | Rabbit anti-Goat IgG-HRP | 100ul,500ul,1ml | |
| 一抗孵育 | abs830001 | Anti- GST | 50ul,100ul | |
| abs830002 | Anti- His Tag抗體 | 50ul,100ul | ||
| abs830003 | Anti- His(C-terminal Specific) 抗體 | 50ul,100ul | ||
| abs830004 | Anti- Myc 抗體 | 50ul,100ul | ||
| abs830005 | Anti- DYKDDDDK | 50ul,100ul | ||
| abs830006 | Anti- HA 抗體 | 50ul,100ul | ||
| abs830008 | Anti-V5,mAb | 50ul,100ul | ||
| abs830010 | Anti- GST,HRP | 50ul | ||
| abs830011 | Anti- His Tag (HRP) 抗體 | 50ul | ||
| abs830012 | Anti- His(C-terminal Specific) (HRP) 抗體 | 50ul | ||
| abs830013 | Anti- Myc (HRP) 抗體 | 50ul | ||
| abs830014 | Anti- DYKDDDDK,HRP | 50ul | ||
| abs830015 | Anti- HA (HRP) 抗體 | 50ul | ||
| abs830016 | Anti- V5 (HRP) 抗體 | 50ul | ||
| abs137956 | C-Myc-Tag Mouse Monoclonal Antibody | 50ug,100ug | ||
| abs137958 | Flag-Tag Mouse Monoclonal Antibody | 50ug,100ug | ||
| 顯色 | ★★★★★ | abs920 | ECL化學(xué)發(fā)光檢測試劑盒 | 2*25ml,2*50ml,2*250ml |
| abs9212 | DAB 顯色試劑盒（棕黃色，WB） | 1kit | ||
| abs9213 | DAB 顯色試劑盒（紫藍(lán)色，WB） | 1kit | ||
| 其他輔助試劑 | abs952 | 10*TBST | 100ml,1L | |
| abs9152 | Tween-20 | 500ml | ||
| ★★ | abs954 | WB一抗二抗稀釋液 | 100ml | |
| abs969 | Western一抗二抗去除液（中性） | 250ml | ||
| abs968 | Western一抗二抗去除液（弱堿性） | 250ml | ||
| abs967 | Western一抗二抗去除液（強(qiáng)堿性） | 250ml | ||
| 新手* | ★★★★★ | abs926 | WB solution base kit（rabbit） | 1kit |
| ★★★★★ | abs927 | WB solution base kit（mouse） | 1kit | |
| ★★★★★ | abs928 | WB solution base kit（goat） | 1kit |
Absin特色產(chǎn)品線： WB相關(guān)：ECL發(fā)光液、預(yù)染marker、預(yù)制膠；IHC相關(guān)：二抗試劑盒、組化筆；IP/CoIP試劑盒；激動(dòng)劑/抑制劑；血清、BSA、蛋白酶K、CTB、TTX、CEE；凋亡試劑盒；呼吸爆發(fā)試劑盒；ELISA試劑盒；重組蛋白；抗體: 二抗、標(biāo)簽抗體、對(duì)照抗體；定制服務(wù)(抗體/多肽/蛋白/標(biāo)記/檢測)... |
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