CD45（LCA）白細胞共同抗原

CD45(LCA)白細胞共同抗原

廣州健侖生物科技有限公司

白細胞共同抗原主要位于白細胞表面，包括 T、B 淋巴細胞、多形核白細胞、單核細胞等，因此是區(qū)別淋巴瘤/白血病和非造血組織腫瘤如未分化小細胞癌、小圓細胞肉瘤的特異性參考依據(jù)。

我司還提供其它進口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【產(chǎn)品介紹】

細胞定位：細胞膜

克隆號：2B11&PD7/26

同型：IgG1/K

適用組織：石蠟/冰凍

陽性對照：扁桃體/小細胞淋巴瘤

抗原修復(fù)：熱修復(fù)（EDTA）

抗體孵育時間：30-60min

CD45(LCA)白細胞共同抗原

理想細胞轉(zhuǎn)染方法，應(yīng)該具有轉(zhuǎn)染效率高、細胞毒性小等優(yōu)點。病毒介導(dǎo)的轉(zhuǎn)染技術(shù)，是目前轉(zhuǎn)染效率zui高的方法，同時具有細胞毒性很低的優(yōu)勢。但是，病毒轉(zhuǎn)染方法的準備程序復(fù)雜，常常對細胞類型有很強的選擇性，在一般實驗室中很難普及。其它物理和化學(xué)介導(dǎo)的轉(zhuǎn)染方法，則各有其特點。
需要指出的一點，無論采用哪種轉(zhuǎn)染技術(shù)，要獲得*的轉(zhuǎn)染結(jié)果，可能都需要對轉(zhuǎn)染條件進行優(yōu)化。影響轉(zhuǎn)染效率的因素很多，從細胞類型、細胞培養(yǎng)條件和細胞生長狀態(tài)，到轉(zhuǎn)染方法的操作細節(jié)，都需要考慮。
一、細胞傳代
1. 試驗準備：200ul/1mlTip頭各一盒(以上物品均需高壓滅菌)，酒精棉球，廢液缸，試管架，微量移液器，記號筆，培養(yǎng)皿，離心管。
2. 棄掉培養(yǎng)皿中的培養(yǎng)基，用1ml的PBS溶液洗滌兩次。
3. 用Tip頭加入1ml Trypsin液，消化1分鐘(37℃，5%CO2 )。用手輕拍培養(yǎng)瓶壁，觀察到細胞*從壁上脫落下來為止。
4. 加入1ml的含血清培養(yǎng)基終止反應(yīng)。
5. 用Tip頭多次吹吸，使細胞*分散開。
6. 將培養(yǎng)液裝入離心管中，1000rpm離心5min。
7. 用培養(yǎng)液重懸細胞，細胞計數(shù)后選擇0.8X106個細胞加入一個35mm培養(yǎng)皿。
8. 將合適體積*培養(yǎng)液加入離心管中，混勻細胞后輕輕加入培養(yǎng)皿中，使其均勻分布。
9. 將培養(yǎng)皿轉(zhuǎn)入CO2培養(yǎng)箱中培養(yǎng)，第二天轉(zhuǎn)染。

CD45(LCA)白細胞共同抗原

我司還提供其它進口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】     歐

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【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

The ideal cell transfection method should have the advantages of high transfection efficiency and low cytotoxicity. Virus-mediated transfection is currently the most efficient transfection method, with low cytotoxicity advantages. However, the procedure for preparation of virus-based transfection is complex and is often highly selective for cell types and is not readily available in the general laboratory. Other physical and chemical-mediated transfection methods have their own characteristics.
It should be pointed out that no matter what kind of transfection technique is used, the optimal transfection results may need to be optimized for transfection conditions. Factors that affect transfection efficiency are numerous and need to be taken into account in terms of cell type, cell culture conditions, and cell growth status, as well as the operational details of the transfection method.
First, the cell passage
1. Test Preparation: 200ul / 1mlTip each one box (above items are autoclaved), alcohol cotton balls, waste tanks, test tube rack, micropipette, marker, petri dishes, centrifuge tubes.
2. Discard the culture medium in the Petri dish and wash twice with 1 ml of PBS solution.
3. Add 1 ml of Trypsin solution to Tip for 1 minute (37 ° C, 5% CO2). Raise the bottle wall with a hand and observe that the cell has compley come off the wall.
4. Add 1ml of serum-containing medium to stop the reaction.
Tip Tip repeatedly suction, the cells compley dispersed.
6. The culture medium into the centrifuge tube, 1000rpm centrifuge 5min.
7. Resuspend the cells in culture and select 0.8 × 10 6 cells to add to a 35 mm Petri dish.
8. The appropriate volume of complete medium was added to the centrifuge tube, mix the cells gently added to the Petri dish, to its uniform distribution.
9. Petri dishes into CO2 incubator culture, the next day transfected.