C4d 補(bǔ)體成分4d（兔多克隆抗體）

廣州健侖生物科技有限公司

補(bǔ)體(complement，C)是存在于正常人和動(dòng)物血清與組織液中的一組經(jīng)活化后具有酶活性的蛋白質(zhì)。早在19世紀(jì)末Bordet即證實(shí)，新鮮血液中含有一種不耐熱的成分，可輔助和補(bǔ)充特異性抗體，介導(dǎo)免疫溶菌、溶血作用，故稱為補(bǔ)體。補(bǔ)體是由30余種可溶性蛋白、膜結(jié)合性蛋白和補(bǔ)體受體組成的多分子系統(tǒng)，故稱為補(bǔ)體系統(tǒng)（complement system）。根據(jù)補(bǔ)體系統(tǒng)各成分的生物學(xué)功能，可將其分為補(bǔ)體固有成分、補(bǔ)體調(diào)控成分和補(bǔ)體受體（CR）。

我司還提供其它進(jìn)口或國(guó)產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測(cè)、食品安全檢測(cè)等試劑盒以及日本生研細(xì)菌分型診斷血清、德國(guó)SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【產(chǎn)品介紹】

細(xì)胞定位：細(xì)胞漿/細(xì)胞膜

同型：IgG2b

適用組織：石蠟/冰凍

陽(yáng)性對(duì)照：淋巴結(jié)/急性腎移植排斥反應(yīng)/扁桃體

抗原修復(fù)：熱修復(fù)（EDTA）

抗體孵育時(shí)間：30-60min

C4d 補(bǔ)體成分4d（兔多克隆抗體）

在酵母中進(jìn)行功能互補(bǔ)實(shí)驗(yàn)無(wú)疑是一種研究人類基因功能的捷徑。如果一個(gè)功能未知的人類基因可以補(bǔ)償酵母中某個(gè)具有已知功能的突變基因，則表明兩者具有相似的功能。而對(duì)于一些功能已知的人類基因，進(jìn)行功能互補(bǔ)實(shí)驗(yàn)也有重要意義。例如與半乳糖血癥相關(guān)的三個(gè)人類基因GALK2（半乳糖激酶）、GALT(UDP-半乳糖轉(zhuǎn)移酶）和GALE(UDP-半乳糖異構(gòu)酶）能分別補(bǔ)償酵母中相應(yīng)的GAL1、GAL7、GAL10基因突變。在進(jìn)行互補(bǔ)實(shí)驗(yàn)以前，人類和酵母的乳糖代謝途徑都已十分清楚，對(duì)有關(guān)幾種酶的活性檢測(cè)法也十分健全，并已獲得其純品，可以進(jìn)行一系列生化分析。隨著人類三個(gè)半乳糖血癥相關(guān)基因的克隆分離成功，功能互補(bǔ)實(shí)驗(yàn)成為可能，從而在遺傳學(xué)水平進(jìn)一步確證了人類半乳糖血癥相關(guān)基因與酵母基因的保守性。人們又將這一成果予以推廣，利用酵母系統(tǒng)進(jìn)行半乳糖血癥的檢測(cè)和基因治療，如區(qū)別真正的突變型和遺傳多態(tài)性，在酵母中模擬多種突變型的組合表型，或篩選基因內(nèi)或基因間的抑制突變等。這些方法也同樣適用于其它遺傳病的研究。
利用異源基因與酵母基因的功能，還能使酵母成為其它生物新基因的篩查工具。通過(guò)使用特定的酵母基因突變株，對(duì)人類cDNA表達(dá)文庫(kù)進(jìn)行篩選，從而獲得互補(bǔ)的克隆。如Tagendreich等利用酵母的細(xì)胞分裂突變型（cdcmutant）分離到多個(gè)在人類細(xì)胞有絲分裂過(guò)程中起作用的同源基因。利用此方法，人們還克隆分離到了農(nóng)作物、家畜和家禽等的多個(gè)新基因。
為了充分發(fā)揮酵母作為模式生物的作用，除了發(fā)展酵母生物信息學(xué)和健全異源基因在酵母中進(jìn)行功能互補(bǔ)的研究方法外，通過(guò)建立酵母zui小的基因組也是一個(gè)可行的途徑。酵母zui小的基因組是指所有明顯豐余的基因減少到允許酵母在實(shí)驗(yàn)條件下的合成培養(yǎng)基中生長(zhǎng)的zui小數(shù)目。人類cDNA克隆與酵母中功能已知基因缺陷型進(jìn)行遺傳互補(bǔ)可以確定人類新基因的功能，但是這種互補(bǔ)實(shí)驗(yàn)會(huì)受到酵母基因組中其它豐余基因的影響。如果構(gòu)建的酵母zui小基因組中所保留的基因可以被人類或者病毒的DNA序列*替換，那么替換后的表型將*取決于外源基因，這將成為一種篩選抗癌和抗病抗原抗體物的分析系統(tǒng)。

C4d 補(bǔ)體成分4d（兔多克隆抗體）

我司還提供其它進(jìn)口或國(guó)產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測(cè)、食品安全檢測(cè)等試劑盒以及日本生研細(xì)菌分型診斷血清、德國(guó)SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【公司名稱】 廣州健侖生物科技有限公司
【市場(chǎng)部】    楊永漢

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【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

Functional complementation in yeast is undoubtedly a shortcut to studying the function of human genes. If a human gene with unknown function can compensate for a certain mutant gene with known function in yeast, the two genes have similar functions. However, for some human genes with known functions, it is also of great significance to perform functional complementation experiments. For example, the three human genes GALK2 (galactokinase), GALT (UDP-galactosyltransferase) and GALE (UDP-galactose isomerase) associated with galactosemia are able to compensate for the corresponding GAL1, GAL7, GAL10 gene mutation. Before performing complementary experiments, the lactose metabolic pathways in humans and yeast are well understood and the assay for the activity of several enzymes involved is well established and has acquired its pure product for a series of biochemical analyzes. With the successful cloning and isolation of three galactose related genes in human, functional complementation experiments become possible, which further confirms the conservatism of human galactose related gene and yeast gene at the level of genetics. In turn, this work is being promoted to detect and genetically treat galactosemia using yeast systems, such as distinguishing between true mutants and genetic polymorphisms, in yeast to model multiple mutant combinatorial phenotypes, or by screening Gene or inter-gene suppression mutations. These methods are equally applicable to other genetic diseases.
The use of heterologous genes and yeast genes can also make yeast a screening tool for new genes in other organisms. Human cDNA expression libraries are screened using specific yeast gene mutants to obtain complementary clones. For example, Tagendreich et al. Used yeast cell division mutants (cdcmutants) to isolate a number of homologous genes that play a role in mitosis in human cells. With this approach, many new genes have also been cloned and isolated from crops, livestock and poultry.
In order to give full play to the role of yeast as a model organism, in addition to the development of yeast bioinformatics and the study of functional complementation of heterologous genes in yeast, it is also a viable approach to establish the smallest genome of yeast. The smallest yeast genome refers to the minimum number of genes that are significantly redundant to allow growth of the yeast in synthetic medium under experimental conditions. Genetic complementation of human cDNA clones with genetic defects in yeast that have known genetic defects can determine the function of human new genes, but such complementation experiments can be influenced by other excess genes in the yeast genome. If the gene retained in the constructed minimal genome of yeast can be compley replaced by the human or viral DNA sequence, the replaced phenotype will be compley dependent on the foreign gene, which will serve as a screen for anti-cancer and anti-disease antigens Analysis system.