花菁染料cy5 cy3花菁染料cy7花菁染料生產(chǎn)廠家
Cyanine菁染料是近紅外熒光染料中常用的一類熒光染料，細(xì)胞和組織的自發(fā)熒光在近紅外波段小，能提供更高的特異性和靈敏度，菁染料是二個非區(qū)域電荷的氮原子通過聚合搭橋的方式形成的一種分子，以下圖片詮釋：
由于Cy染料的結(jié)構(gòu)式特點，他們往往有著*的消選光系數(shù)，數(shù)值高達(dá)100,000 Lmol-1cm-1
Cy系列的熒光染料我們一般分為磺酸化菁染料和非磺酸化菁染料non-sulfonated cyanines, and sulfonated cyanines.他們的光譜性質(zhì)基本相同，和蛋白 小分子修飾反應(yīng)也幾乎是一樣的，不同的在于他們的溶解性，非磺酸化菁染料是脂溶性的，磺酸化菁染料是水溶性的，結(jié)構(gòu)式上也會有些不同。
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Non-sulfonated cyanines非磺酸化菁染料包括以下：
Cy3.5 alkyne、Cy3 azide、Cy3 carboxylic acid、Cy3 NHS ester、Cy5 amine、Cy5 maleimide
Cyanine3 alkyne azide carboxylic acid hydrazide maleimide NHS ester
Cyanine3.5 alkyne azide carboxylic acid hydrazide maleimide NHS ester
Cyanine5 alkyne azide carboxylic acid hydrazide maleimide NHS ester amine
Cyanine5.5 alkyne azide carboxylic acid hydrazide maleimide NHS ester amine
Cyanine7 alkyne azide carboxylic acid hydrazide maleimide NHS ester amine
Cyanine7.5 alkyne azide carboxylic acid hydrazide maleimide NHS ester amine
大多數(shù)非磺酸化菁染料都必須在有機相里去跟蛋白、多肽、抗體及小分子材料進(jìn)行下一步的反應(yīng)。
花菁染料cy5 cy3花菁染料cy7花菁染料生產(chǎn)廠家【5mg/10mg/20mg/25mg】
Sulfonated cyanine磺酸化菁染料包括以下：
Sulfo-Cy5 NHS ester, Sulfo-Cy3 maleimide, Sulfo-Cy7 azide, Sulfo-Cy5 carboxylic acid
Sulfo Cyanine3 alkyne azide carboxylic acid hydrazide maleimide NHS ester
Sulfo Cyanine3.5 alkyne azide carboxylic acid hydrazide maleimide NHS ester
Sulfo Cyanine5 alkyne azide carboxylic acid hydrazide maleimide NHS ester amine
Sulfo Cyanine5.5 alkyne azide carboxylic acid hydrazide maleimide NHS ester amine
Sulfo Cyanine7 alkyne azide carboxylic acid hydrazide maleimide NHS ester amine
Sulfo Cyanine7.5 alkyne azide carboxylic acid hydrazide maleimide NHS ester amine
雖然非磺酸化菁染料和磺酸化菁染料有著相同的熒光特性，但是在具體使用上還是有一些差異性的，非磺酸化菁染料必須溶解在有機溶劑中如DMF或DMSO中使用，并加入緩沖水溶液buffers來使用，磺酸化菁染料溶解在水中，整個使用過程*在水相中進(jìn)行。
Most derivatives of non-sulfonated cyanines (except for hydrochlorides of hydrazides and amines) have low aqueous solubility. When these molecules are used for biomolecule labeling, use of organic co-solvent (5-20% of DMF or DMSO) is necessary for efficient reaction. Cyanine dye should be dissolved in organic solvent first, and added to a solution of biomolecule (protein, peptide, amino-labeled DNA) in appropriate aqueous buffer. When conjugation takes place efficiently, the dye reacts before it precipitates.
Fluorescent properties of non-sulfonated cyanines have little dependence on solvent and surrounding. Absorbance and fluorescence spectra of non-sulfonated cyanine dyes are plotted below.
花菁染料cy5 cy3花菁染料cy7花菁染料生產(chǎn)廠家【5mg/10mg/20mg/25mg】
公司其他相關(guān)產(chǎn)品：
Cyanine3 alkyne
Cy3 alkyne
Cyanine3 azide
Cy3 azide
Cy3 N3
Cyanine3 carboxylic acid 1144107-76-5
Cy3 carboxylic acid
Cyanine3 maleimide
Cy3 maleimide
Cyanine3 NHS ester 1032678-38-8
Cy3 NHS ester
Cyanine5 alkyne
Cyanine5 amine
Cy5 amine
Cy5 NH2
Cyanine5 azide
Cy5 N3
Cyanine5 Boc-hydrazide
Cyanine5 carboxylic acid
Cyanine5 hydrazide
Cyanine5 maleimide
Cy5 maleimide
Cyanine5 NHS ester 1032678-42-4
Cy5 NHS ester
Cyanine7 amine 1650635-41-8
Cy7 amine
Cy7 NH2
Cyanine7 azide
Cy7 azide
Cyanine7 carboxylic acid
Cyanine7 maleimide
Cy7 maleimide
Cyanine7 NHS ester
Sulfo-Cyanine 5 NHS ester
Sulfo-Cy 5 NHS ester
Sulfo-Cyanine3 azide
Sulfo-Cy3 azide
Sulfo-Cyanine3 carboxylic acid
Sulfo-Cyanine3 maleimide
Sulfo-Cy3 maleimide
Sulfo-Cyanine3 NHS ester
Sulfo-Cy3 NHS ester
Sulfo-Cyanine5 alkyne
Sulfo-Cyanine5 azide
Sulfo-Cy5 azide
Sulfo-Cyanine5 carboxylic acid 1121756-16-8
Sulfo-Cyanine5 maleimide
Sulfo-Cy5 maleimide
Sulfo-Cyanine7 azide
Sulfo-Cyanine7 carboxylic acid
Sulfo-Cyanine7 NHS ester
Sulfo-Cy7 NHS ester
Cyanine3 alkyne
Cy3 alkyne
Cyanine3 azide
Cy3 azide
Cy3 N3
Cyanine3 carboxylic acid 1144107-76-5
Cy3 carboxylic acid
Cyanine3 hydrazide
Cyanine3 maleimide
Cy3 maleimide
Cyanine3 NHS ester 1032678-38-8
Cy3 NHS ester
Cyanine5 alkyne
Cyanine5 amine
Cy5 amine
Cy5 NH2
Cyanine5 azide
Cy5 N3
Cyanine5 carboxylic acid
Cyanine5 hydrazide
Cyanine5 maleimide
Cy5 maleimide
Cyanine5 NHS ester 1032678-42-4
Cy5 NHS ester
Cyanine7 amine 1650635-41-8
Cy7 amine
Cy7 NH2
Cyanine7 azide
Cy7 azide
Cyanine7 carboxylic acid
Cyanine7 maleimide
Cy7 maleimide
Cyanine7 NHS ester
Cy7 NHS ester
Sulfo-Cyanine 5 NHS ester
Sulfo-Cy 5 NHS ester
Sulfo-Cyanine3 azide
Sulfo-Cy3 azide
Sulfo-Cyanine3 carboxylic acid
Sulfo-Cyanine3 maleimide
Sulfo-Cy3 maleimide
Sulfo-Cyanine3 NHS ester
Sulfo-Cy3 NHS ester
Sulfo-Cyanine5 alkyne
Sulfo-Cyanine5 azide
Sulfo-Cy5 azide
Sulfo-Cyanine5 carboxylic acid 1121756-16-8
Sulfo-Cyanine5 maleimide
Sulfo-Cy5 maleimide
Sulfo-Cyanine7 azide
Sulfo-Cyanine7 carboxylic acid
Sulfo-Cyanine7 NHS ester
Sulfo-Cy7 NHS ester
