Biomomentum多軸機械測試儀Mach-1應(yīng)用：

Automated Indentation Demonstrates Structural Stiffness of Femoral Articular Cartilage and Temporomandibular Joint Mandibular Condylar Cartilage Is Altered in FgF2KO Mice

Paige S. Woods, Alyssa A. Morin, Po-Jung Chen, Sarah Mahonski, Liping Xiao, Marja Hurley, Sumit Yadav, and Tannin A. Schmidt

CARTILAGE. October 2020. doi:10.1177/1947603520962565

Objective

Employ an automated indentation technique, using a commercially available machine, to assess the effect of fibroblast growth factor 2 (FGF2) expression on structural stiffness over the surface of both murine femoral articular cartilage (AC) and temporomandibular joint (TMJ) mandibular condylar cartilage (MCC).

 

Design

Experiments were performed using 3-month-old female homozygote Fgf2KO mice with wild type (WT) littermates. After euthanization, isolated mandibles and hindlimbs were either processed for histology or subjected to automated indentation on a Biomomentum Mach-1 v500csst with a 3-axis motion controller in a phosphate buffered saline bath using a 0.3 mm spherical tip indenter. The effect of indentation depth on normal force was characterized, then structural stiffness was calculated and mapped at multiple positions on the AC and MCC.

Results

Automated indentation of the AC and TMJ MCC was successfully completed and was able to demonstrate both regional variation in structural stiffness and differences between WT and Fgf2KO mice. Structural stiffness values for Fgf2KO AC were significantly smaller than WT at both the medial/anterior (P < 0.05) and medial/posterior (P < 0.05) positions. Global Fgf2KO also lead to a decrease in MCC thickness of the TMJ compared with WT (P < 0.05) and increased structural stiffness values for Fgf2KO at both the posterior and anterior location (P < 0.05).

 

Conclusions

Biomomentum多軸機械測試儀Mach-1應(yīng)用：自動壓痕表明FgF2KO小鼠股骨軟骨的結(jié)構(gòu)剛度和顳下頜關(guān)節(jié)下頜Con突軟骨發(fā)生改變。

采用自動壓痕技術(shù)，使用市售機器評估成纖維細(xì)胞生長因子2（FGF2）表達(dá)對鼠股骨關(guān)節(jié)軟骨（AC）和顳下頜關(guān)節(jié)（TMJ）下頜con突軟骨表面的結(jié)構(gòu)剛度的影響（我的客戶中心（MCC）。設(shè)計使用具有野生型（WT）同窩仔的3個月大的雌性純合子Fgf2KO小鼠進(jìn)行實驗。對分離出的下頜骨和后肢進(jìn)行組織學(xué)處理，或在帶有3-軸運動控制器的Biomomentum Mach-1 v500csst上使用0.3毫米球形尖壓頭在磷酸鹽緩沖鹽水浴中進(jìn)行自動壓痕。表征了壓痕深度對法向力的影響，然后計算結(jié)構(gòu)剛度并將其映射到AC和MCC的多個位置。結(jié)果AC和TMJ MCC的自動壓痕已成功完成，并且能夠證明結(jié)構(gòu)剛度的區(qū)域差異以及WT和Fgf2KO小鼠之間的差異。Fgf2KO AC的結(jié)構(gòu)剛度值在內(nèi)側(cè)/前側(cè)（P <0.05）和內(nèi)側(cè)/后側(cè)（P <0.05）位置均顯著小于WT。與WT相比，整體Fgf2KO還會導(dǎo)致TMJ的MCC厚度減?。≒ <0.05），并且在后部和前部位置的Fgf2KO的結(jié)構(gòu)剛度值都會增加（P <0.05）。