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重組Anti-Ki67抗體[SP6],兔單克隆抗體[SP6] to Ki67,Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply.

產(chǎn)品名稱
Anti-Ki67抗體[SP6]
參閱全部 Ki67 一抗
描述
兔單克隆抗體[SP6] to Ki67
宿主
Rabbit
經(jīng)測(cè)試應(yīng)用
適用于: Flow Cyt (Intra),IHC-P,WB,mIHC,ICC/IFmore details
種屬反應(yīng)性
與反應(yīng): Mouse, Rat, Human
預(yù)測(cè)可用于: Common marmoset
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
表位
C-terminus
陽(yáng)性對(duì)照

WB: HeLa cell lysate. IHC-P: Human tonsil, rat and mouse spleen tissues. ICC/IF: HeLa and HAP1 cells. Flow Cyt (intra): HAP1 cells.

常規(guī)說(shuō)明
ab16667 was switched from a hybridoma to recombinant production method on 24th October 2019.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information see here.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

形式
Liquid
存放說(shuō)明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
存儲(chǔ)溶液
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS
純度
Protein A purified
克隆
單克隆
克隆編號(hào)
SP6
同種型
IgG

Abpromise™承諾保證使用ab16667于以下的經(jīng)測(cè)試應(yīng)用

“應(yīng)用說(shuō)明"部分下顯示的僅為推薦的起始稀釋度；實(shí)際最佳的稀釋度/濃度應(yīng)由使用者檢定。

應(yīng)用	Ab評(píng)論	說(shuō)明
Flow Cyt (Intra)		1/1000. ab172730, Rabbit monoclonal isotype, is suitable for use as an isotype control with this antibody.
IHC-P	(62)	1/200. Antigen retrieval: Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). Primary antibody condition: primary antibody incubation overnight at +4°C is recommended.
WB	(3)	Use at an assay dependent concentration. Predicted molecular weight: 358 kDa.
mIHC		Use at an assay dependent concentration.
ICC/IF	(22)	1/250. If fixing cells in 4% PFA (20 min, room temp), it is recommended to permeabilized cells with 0.1% Triton-X for 5 min.

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