on silanized glass surfaces during flow through a linear channel

at flow rates of 0.11 mL/min using confocal microscopy. We

assemble layers of organosilanes on glass and track the position

and orientation of bacteria deposited on these surfaces during

flow with high spatial resolution. We find that a metric based on

the degree of the surface-tethered motion of bacteria driven by

flagella is inversely correlated with deposition rate, whereas

conventional surface characterizations, such as surface energy or

water contact angle, are uncorrelated. Furthermore, the

likelihood that an initially moving bacterium becomes immobilized increases with increasing deposition rate. Our results

suggest that the chemistry and arrangement of silane molecules on the surface influence the transition from transient to

irreversible attachment by favoring different mechanisms used by bacteria to attach to surfaces.