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沙門氏菌培養(yǎng)基的配置過程

時(shí)間:2011/11/7閱讀:547
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 名稱:沙門氏菌培養(yǎng)基

用 途:用于腸道致病菌特別是沙門氏菌和志賀氏菌的選擇性分離培養(yǎng)
原 理:蛋白胨、牛肉膏粉提供碳源、氮源、維生素和礦物質(zhì);乳糖、蔗糖為可發(fā)酵的糖類;去氧膽酸鈉和檸檬酸鈉抑制革蘭氏陽性菌及大多數(shù)的大腸菌群和變形桿菌,但不影響沙門氏菌的生長;硫代硫酸鈉和檸檬酸鐵銨用于檢測硫化氫的產(chǎn)生,使菌落中心呈黑色;中性紅為ph指示劑,發(fā)酵糖產(chǎn)酸的菌落呈紅色,不發(fā)酵糖的菌落為無色;瓊脂是培養(yǎng)基的凝固劑。
配方(每升):胨 20.0g乳糖 10.0g牛肉膏粉3.0g蔗糖 10.0g去氧膽酸鈉 1.0g硫代硫酸鈉2.3g檸檬酸鈉 1.0g檸檬酸鐵銨1.0g中性紅 0.03g瓊脂 16.0gzui終ph 7.2±0.1
使用方法:
1.稱取本品63.3g,加入蒸餾水或去離子水1 l,攪拌加熱煮沸至*溶解,待冷至50℃左右,在無菌環(huán)境中,傾注滅菌平皿,待凝固后,備用。
2.用接種環(huán)取增菌液一環(huán),劃線接種于平板上。
3.將平板放入恒溫培養(yǎng)箱中,36±1℃培養(yǎng)18~24h。
4.觀察結(jié)果。

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