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防止培養(yǎng)基長霉的方法

時間:2011/11/7閱讀:567
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當環(huán)境溫度不適應果蠅生長時(溫度低于18℃) ,培養(yǎng)基接種后的7d內,特別容易長霉。
要避免長霉,應注意:
①培養(yǎng)果蠅的器皿要嚴格消毒。培養(yǎng)瓶洗干凈后,自然涼干, 再與棉塞一道進行高溫干燥滅菌(120℃, 30min) 。 
②培養(yǎng)基營養(yǎng)要全面。果蠅培養(yǎng)基的種類很多,本實驗室用的是玉米培養(yǎng)基,配方如下:水750mL煮開,加瓊脂6. 2g,待瓊脂溶解后加白糖62.5g,攪拌溶解后加調成糊狀的玉米糊(玉米粉82. 5g,用冷水調成糊狀) ,煮開2~3min,加酵母粉7g,加熱1~2min,攪拌均勻后,移去火源,加丙酸2mL (起殺菌防霉的作用) 。培養(yǎng)基稍稍冷卻后,倒入已滅菌的培養(yǎng)基瓶中,盡量不要讓培養(yǎng)基粘在瓶壁上,塞好棉塞,放入仍有余熱的干燥箱中將瓶壁上的水蒸干。
③接種時消好毒。在無菌室內接種, 如沒有無菌室, 在接種前用75%酒精棉球擦雙手一遍。接種后,放入適宜果蠅生長溫度的培養(yǎng)箱中培養(yǎng)果蠅(溫度20~25℃) ,一旦下一代成蠅孵出來后,培養(yǎng)基就不易長霉。
長霉后的處理方法:
①若培養(yǎng)基長霉后,由于霉菌孢子漂浮于培養(yǎng)瓶的整個空間,包括果蠅身上,如將此果蠅接種到新的培養(yǎng)基中,大約經過4 ~5d,培養(yǎng)基將會重新長出霉菌來,遇到該問題,解決的方法只有兩種:一是不再接種這種長霉菌果蠅,重新接種未長霉的果蠅;二是采取措施殺死果蠅身上的霉菌孢子。 
②殺霉菌方法為:將帶有霉菌孢子的成蠅倒入空培養(yǎng)瓶中,棉塞上倒適量的75%酒精,再將瓶口塞緊,利用酒精具有揮發(fā)性,揮發(fā)后的酒精充滿整個瓶內,從而將果蠅身上的霉孢子殺死。
在操作上應注意兩點:一是倒在棉塞上的酒精量要合適,過多果蠅易醉昏,過少殺死不了霉菌孢子;二是果蠅呆在空瓶內的時間要恰當,以2d為好。

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