AnaSpec-β-淀粉樣蛋白(1-42)在神經(jīng)退行性疾病中的作用機(jī)制

Aβ（1-42）是淀粉樣斑塊的主要成分，在阿爾茨海默病患者大腦的神經(jīng)元中積累。從阿爾茨海默病患者大腦中分離出的淀粉樣肽的生化分析表明，Aβ（1-42）是與老年斑淀粉樣蛋白相關(guān)的主要種類，而Aβ（1-40）在腦血管淀粉樣沉積物中更為豐富。

艾美捷AnaSpec-β-淀粉樣蛋白(1-42)：

貨號：AS-20276

分子量：4514.1

HPLC峰面積百分比：≥95%

肽含量：≥60%

外觀：凍干白色粉末

肽復(fù)溶：使用1.0% NH?OH作為溶劑，隨后用緩沖液（如1X PBS）稀釋。將1.0% NH?OH直接加入凍干肽粉中（加入35-40 µl至0.5 mg肽粉，或70-80 µl至1 mg肽粉）。肽在1.0% NH?OH中不能長期儲(chǔ)存，因此重要的是立即將該溶液用1X PBS或其他緩沖液稀釋至大約1 mg/ml或更低的濃度。輕輕渦旋混合。

儲(chǔ)存：肽在常溫下運(yùn)輸。收到后，將凍干粉末儲(chǔ)存于-20°C或更低溫度。復(fù)溶后的肽應(yīng)分裝到多個(gè)冷凍管中，并儲(chǔ)存于-20°C或更低溫度。避免反復(fù)凍融。

相關(guān)產(chǎn)品：

AS-61322   1 % NH4OH solution - 300 µl

AS-72223   SensoLyte 520 Neprilysin Activity Assay Kit Fluorimetric - 1 kit

AS-72214   SensoLyte Thioflavin T ?-Amyloid (1-42) Aggregation Kit - 1 kit

引文：

Importance of the Dimethylamino Functionality on a Multifunctional Framework for Regulating Metals, Amyloid-β, and Oxidative Stress in Alzheimer’s Disease

Novel Molecular Insights into Classical and Alternative Activation States of Microglia as Revealed by Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)-based Proteomics

Tailoring the antibody response to aggregated A? using novel Alzheimer-Vaccines

LC3 overexpression reduces Aβ neurotoxicity through increasing α7nAchR expression and autophagic activity in neurons and mice

參考：

Epitope mapping and neuroprotective properties of a human single chain FV antibody that binds an internal epitope of amyloid-beta 1-42

Intracellular accumulation of β-amyloid1–42 in neurons is facilitated by the α7 nicotinic acetylcholine receptor in Alzheimer’s disease

Soluble Amyloid Aβ-(1–40) Exists as a Stable Dimer at Low Concentrations

艾美捷科技是AnaSpec的中國代理商，為科研工作者提供優(yōu)質(zhì)的產(chǎn)品與服務(wù)。