3D單分子熒光成像系統(tǒng)-SAFe 360

3D單分子熒光成像系統(tǒng)-SAFe 360簡介：

SAFe 360是法國abbelight公司推出的款基于單分子定位的顯微成像（SMLM）的新型3D單分子成像系統(tǒng)，它*的DAISY技術(shù)整合了散光技術(shù)和超臨界角光技術(shù)，能夠很大的提高定位精度，xyz三軸定位精度高達15nm，可以提供高清晰三維亞細胞結(jié)構(gòu)圖像，支持同時多四色成像，可以用于細胞納米三維成像，觀測高清晰亞細胞器結(jié)構(gòu)，實時研究不同的結(jié)構(gòu)功能蛋白的共定位信息，在單分子水平研究分子動力學(xué)反應(yīng)以及細胞間的相互作用等。

3D單分子熒光成像系統(tǒng)-SAFe 360設(shè)備參數(shù)

+ 成像模式：PALM、STORM、PAINT、smFRET 、SPT

+ 光源模式：Epi、TIRF、HILO

+ 分辨率：15 nm的XYZ軸分辨率

+ 超大視野：200 × 200 μm2的視野

+  次可同時采集1.2 μm深度圖像信息

+  圖像深度：10 μm

+  實時漂移矯正

+  四色同時成像

+  活細胞成像模式

配套試劑

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發(fā)表文章

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[13] Culley, Sian, et al. "Quantitative mapping and minimization of super-resolution optical imaging artifacts." Nature methods 15.4 (2018): 263.

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[15] Cabriel, Clément, et al. "Aberration-accounting calibration for 3D single-molecule localization microscopy." Optics letters 43.2 (2018): 174-177.

[16] Bouissou, Ana?s, et al. "Podosome force generation machinery: a local balance between protrusion at the core and traction at the ring." ACS nano 11.4 (2017): 4028-4040.

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