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【ACE Excel C18-PFP色譜柱】雞蛋中的氨基糖苷類分析
Aminoglycosides in Eggs
UHPLC-MS/MS method for aminoglycosides in eggs
Extraction at low pH, clean-up with WCX SPE cartridge
Key
GNT Gentamicin
TBM Tobramycin
KNM Kanamycin
DHS Dihydrostreptomycin
STR Streptomycin
HGB Higromycin-B
SPT Spectinomycin
ACE Excel C18-PFP, 2μm, 100 x 2.1mm
Gradient analysis
A = 20mM HFBA in H2O/CH3CN (98:2)
B = 20mM HFBA in CH3CN/H2O (98:2)
Time (mins) %B Curve
0 5 -
2 15 6
4.5 19 6
5.5 19.5 8
6 22 6
7 35 6
9 48 8
9.5 5 6
Flow rate: 0.4ml/min
Column temperature: 40°C
Positive ESI MRM (transitions as shown)