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Insulin (INS) 抗體

抗原
Insulin (INS)
別名 ins1, xins, ins1-a, Insulin, IDDM2, ILPR, IRDN, MODY10, AA986540, Ins-2, InsII, Mody, Mody4, proinsulin, zgc:109842
適用
 其他選擇Human, Pig (Porcine)
宿主
 其他選擇Mouse
克隆類型 (克隆位點)
單克隆 (2D11-H5)
標記
 其他選擇非結合性
應用范圍
 其他選擇Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
[Pubmed] 1 reference available
規(guī)格 0.1 mg
發(fā)貨至 中國 (更改)
發(fā)貨時間 29至35個工作日

Insulin (INS) 抗體

產(chǎn)品細節(jié)

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免疫原 Purified human Insulin coupled to Bovine Serum Albumin (BSA).
序列 Human Proinsulin (precursor) 21 aa A chain 90-110. 30 aa B chain 25-54. 1 MALWMRLLPL LALLALWGPD PAAAFVNQHL CGSHLVEALY LVCGERGFFY TPKTRREAED 61 LQVGQVELGG GPGAGSLQPL ALEGSLQKRG IVEQCCTSIC SLYQLENYCN
克隆位點 2D11-H5
亞型 IgG1
特異性 This monoclonal 2D11.H5 antibody recognizes a polypeptide which is identified as Insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. This protein A purified Mouse monoclonal antibody reacts specifically with Insulin from Human and Swine sources. Cross reactivity with Insulin from Mouse and Rat does not occur. Cross reactivity with Insulin from other sources has not been determined.
Cellular Localization: Cytoplasmic.Cellular Localization:
純化方法 Protein A Chromatography

Insulin (INS) 抗體目標詳細情況

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別名 Insulin
背景 Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-peptide basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain acids. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as β-cell and tumor (insulinoma) markers.Synonyms: INS
分子量 6 kDa
基因ID 3630
NCBI Accession NP_000198
UniProt P01308
研究領域 Neurology, Diabetes, Hormones

使用細節(jié)

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應用備注 This antibody is suitable for ELISA, Immunohistochemistry, Immunoblotting andimmunoprecipitation. Insulin has a cytoplasmic localization. Pancreatic tissue or β-cellsfrom islets of Langerhans can be used as a positive control. Generally a 1: 50 to 1: 200 dilution is suitable for most applications. A 30 to 60?minuteincubation time is usually sufficient. However, depending upon the fixation conditions andthe staining system employed, optimal incubation should be determined by the user. Frozen sections of paraffin embedded tissues are recommended for preparations to use forimmunohistochemistry. No special pretreatment is required. Researchers shoulddetermine the optimal dilution for other specific assays and cell types. ELISA: Results of Mab anti-Insulin antibody were tested against human insulin by ELISA. Each well was coated with 0.1g of conjugate. The starting concentration of antibody in thedilution series was 10?μg/mL. Each point on the Y-axis represents a 3-fold dilution. Themidpoint of the titration curve represents approx-imay 5?ng/mL antibody or a 1: 200,000dilution from the stock concentration. HRP conjugated Gt-a- Mouse IgG H&L and TMBsubstrate were used for detection (see figure 1).
限制 For Research Use only

Insulin (INS) 抗體貯存及處理

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濃度 1.0 mg/mL (by UV absorbance at 280 nm)
緩沖液 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride,?pH 7.2, 0.01?% (w/v) Sodium Azide, None
儲存液 Sodium azide
注意事項 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事項 Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
儲存條件 -20 °C
儲存方法 Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.

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