Interleukin 2 Receptor, alpha （IL2RA） 抗體 返回列表頁

Interleukin 2 Receptor, alpha (IL2RA) 抗體

抗原
Interleukin 2 Receptor, alpha (IL2RA)
別名 CD25, HEM45, IDDM10, IL2R, TCGFR, IL2RAC, IL-2RA, Il2r, Ly-43
適用
其他選擇 Rat ((Rattus))
Human(266),Murine (199),Rat ((Rattus)) (73),Monkey (10),Bovine (8),Sheep (Ovine) (6),Pig (Porcine) (2),Chicken (1),Dog (Canine) (1),Rabbit (1)
宿主
其他選擇 Mouse
Mouse(264),Rat (193),Rabbit (64),Sheep (3),Goat (1)
克隆類型 (克隆位點)
單克隆 (OX-39)
標記
其他選擇 PE
PE(64),Biotin (62),FITC (61),APC (30),PE-Cy7 (9),PerCP (8),Alexa Fluor 488 (7),SPRD (6),PE,Cy7 (4),PE-Cy5 (4),PerCP,Cy5.5 (4),Alexa Fluor 700 (2),Allophycocyanin (2),Cy5,PE (2),Cy7,PE (2),HRP (2),PE,Cy5 (2),Alexa Fluor 350 (1),Alexa Fluor 555 (1),Alexa Fluor 647 (1),Alkaline Phosphatase (AP) (1),Cy3 (1),Cy5 (1),Cy5.5 (1),Cy7 (1),DyLight 488 (1),DyLight 550 (1),DyLight 594 (1),DyLight 650 (1),DyLight 680 (1),DyLight 755 (1),Dyomics 647 (1),PE,DY647 (1),PE-Dyomics 647 (1)
應用范圍
其他選擇 Flow Cytometry (FACS)
Flow Cytometry (FACS)(403),Immunoprecipitation (IP) (77),Immunohistochemistry (Frozen Sections) (IHC (fro)) (74),Western Blotting (WB) (70),ELISA (55),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (54),Immunohistochemistry (IHC) (42),Immunofluorescence (IF) (41),Functional Studies (Func) (23),Blocking Antibody (Inhibition) (17),Immunocytochemistry (ICC) (14),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (10),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (10),Enzyme Immunoassay (EIA) (8),ELISA (Capture) (2),ELISA (Detection) (2),Immunoassay (IA) (2),Immunohistochemistry (Acetone-fixed) (IHC (af)) (2),Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2),Neutralization (Neut) (2),Blocking Reagent (BR) (1)
[Pubmed] 1 reference available
規(guī)格 100 tests
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Interleukin 2 Receptor, alpha (IL2RA) 抗體

產(chǎn)品細節(jié)

顯示 隱藏
免疫原 Con A stimulated Rat T cells.
克隆位點 OX-39
亞型 IgG1
特異性 Recognizes the alpha chain of rat CD25, otherwise know as IL-2 receptor alpha, a 55?kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla. This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts (1).
純化方法 Protein G purified immunoglobulin fraction

目標詳細情況

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Interleukin 2 Receptor, alpha (IL2RA) 抗體目標詳細情況

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別名 CD25 / IL2RA
背景 The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternay spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55
基因ID 25704
NCBI Accession NP_037295
UniProt P26897
研究領(lǐng)域 CD Antigens, Surface Receptors of Immune Cells, Cytokines, Receptors, Inflammation

使用細節(jié)

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使用細節(jié)

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應用備注 Flow Cytometry: use 10 μL of neat antibody to label 10^6 cells in 100?μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 For Research Use only

貯存及處理

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貯存及處理

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溶解方式 Reconstitute with 1?mL distilled water.
緩沖液 PBS?pH 7.4 with 0.09?% Sodium Azide as preservative and 1?% BSA as stabilizer.
儲存液 Sodium azide
Interleukin 2 Receptor, alpha (IL2RA) 抗體注意事項 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事項 Avoid repeated freezing and thawing.
儲存條件 -20 °C
儲存方法 Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.
有效期 6 months

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