Alstrom Syndrome 1 （ALMS1） （C-Term） 抗體 返回列表頁

Alstrom Syndrome 1 (ALMS1) (C-Term) 抗體

抗原
Alstrom Syndrome 1 (ALMS1)
別名 ALMS1, ALSS, bbb
抗原表位
其他選擇 C-Term
C-Term(7),Internal Region (6),AA 1200-1250 (2),AA 3400-3450 (1),AA 4104-4167 (1)
適用
其他選擇 Human
Human(19),Dog (Canine) (2),Murine (2),Bovine (1),Rat ((Rattus)) (1)
宿主
其他選擇 Goat
Rabbit(16),Goat (5)
克隆類型
多克隆
實(shí)驗(yàn)類型 Peptide ELISA
應(yīng)用范圍
其他選擇 ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Western Blotting (WB)(15),ELISA (13),Immunohistochemistry (IHC) (8),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4),Immunocytochemistry (ICC) (2),Immunofluorescence (IF) (2),Immunoprecipitation (IP) (2),Enzyme Immunoassay (EIA) (1),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (1),Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
[Pubmed] 1 reference available
規(guī)格 100 μg
發(fā)貨至 中國(guó) (更改)
發(fā)貨時(shí)間 24至29個(gè)工作日

Alstrom Syndrome 1 (ALMS1) (C-Term) 抗體

產(chǎn)品細(xì)節(jié)

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免疫原 Peptide with sequence C-RVTNQLLGRKVPWD, from the C Terminus of the protein sequence according to NP_055935.4.
特異性 The C terminus of ALMS1 shares an 8 amino acid stretch with two other human proteins: hypothetical protein (XP_169104) and ERAL1 (NP_005693). However, this stretch is located internally in these two proteins and so will most likely not cross-react with this antibody. The N terminus was not chosen as the peptide is expected to cyclise during synthesis.
交叉反應(yīng) Murine, Rat ((Rattus)), Dog (Canine), Bovine
純化方法 Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Alstrom Syndrome 1 (ALMS1) (C-Term) 抗體目標(biāo)詳細(xì)情況

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目標(biāo)詳細(xì)情況

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別名 ALMS1
背景 Synonyms: ALMS1, Alstrom syndrome 1, ALSS, KIAA0328, DKFZp686A118, DKFZp686D1828
基因ID 7840, 236266, 297408
NCBI Accession NP_055935
研究領(lǐng)域 Immunology

使用細(xì)節(jié)

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使用細(xì)節(jié)

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應(yīng)用備注 ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave bands at approx 200 kDa and 75 kDa in lysates of Human T cell line Jurkat after 0.3 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 461 kDa according to NP_055935.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
IHC: In paraffin embedded Human Testis shows staining of cytoplasm in spermatocytes. Recommended concentration: 1-3 µg/mL.
限制 For Research Use only

貯存及處理

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貯存及處理

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狀態(tài) Liquid
緩沖液 Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
儲(chǔ)存液 Sodium azide
Alstrom Syndrome 1 (ALMS1) (C-Term) 抗體注意事項(xiàng) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事項(xiàng) Minimize freezing and thawing.
儲(chǔ)存條件 -20 °C
儲(chǔ)存方法 Aliquot and store at -20 °C.

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