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CD28抗體

抗原
CD28 (CD28)
別名 Tp44, CD28RNA
適用
其他選擇 Chicken
Murine(267),Human (201),Rat ((Rattus)) (87),Chicken (45),Bovine (37),Pig (Porcine) (25),Dog (Canine) (13),Monkey (6),Non-Human Primate (6),Primate (6),Turkey (4)
宿主
其他選擇 Mouse
Mouse(220),Hamster (192),Rabbit (73),Rat (33),Syrian Hamster (13),Goat (7)
克隆類型 (克隆位點(diǎn))
單克隆 (AV7)
標(biāo)記
其他選擇 FITC
PE(87),FITC (77),Biotin (71),APC (26),SPRD (8),Allophycocyanin (4),Cy5,PE (4),Cy7,PE (4),HRP (3),PerCP (3),Alexa Fluor 350 (2),Alexa Fluor 488 (2),Alexa Fluor 555 (2),Alexa Fluor 647 (2),Cy3 (2),Cy5 (2),Cy5.5 (2),Cy7 (2),PE,Cy5 (2),PE,Cy7 (2),PerCP,Cy5.5 (2),Alkaline Phosphatase (AP) (1),DyLight 488 (1),DyLight 550 (1),DyLight 594 (1),DyLight 650 (1),DyLight 680 (1),DyLight 755 (1),PE-Cy5 (1)
應(yīng)用范圍
其他選擇 Flow Cytometry (FACS)
Flow Cytometry (FACS)(430),Immunoprecipitation (IP) (110),Western Blotting (WB) (72),ELISA (59),Functional Studies (Func) (52),Immunohistochemistry (Frozen Sections) (IHC (fro)) (34),Immunohistochemistry (IHC) (24),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (20),Immunocytochemistry (ICC) (17),Immunofluorescence (IF) (13),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6),Immunohistochemistry (Acetone-fixed) (IHC (af)) (5),Enzyme Immunoassay (EIA) (2),Immunoassay (IA) (2),Activation (Act) (1)
[Pubmed] 2 references available
規(guī)格 0.5 mg
發(fā)貨至 中國 (更改)
發(fā)貨時(shí)間 23至28個(gè)工作日

CD28抗體

產(chǎn)品細(xì)節(jié)

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產(chǎn)品細(xì)節(jié)

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克隆位點(diǎn) AV7
亞型 IgG1
產(chǎn)品特性 The antibody detects: Chicken CD28, a monomeric molecule of Mr 40-44 kDa with 50 % amino acid sequence identity with mammalian CD28
MAb AV7 also reacts with turkey CD28 as demonstrated by flow cytometry and immunoprecipitation/Western blotting.

目標(biāo)詳細(xì)情況

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目標(biāo)詳細(xì)情況

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別名 CD28
背景 Chicken CD28 antigen is a type I transmembrane monomeric glycoprotein which does not contain the cysteine residues that form disulfide-linked covalent homodimers found in the mammalian counterpart. This may reduce the avidity of binding to individual ligand molecules expected to be present on Ag presenting cells. Peripheral + T cells are CD28-negative.1 Monoclonal antibodies against CD28 have a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A or MAbs against the avian T cell receptor (TCR). Both V1- and V2-expressing cells respond equally well to stimulation with anti-CD28 in combination with PMA. These responses are resistant to cyclosporin A, but inhibited by herbimycin A, suggesting that CD28 employs a signalling pathway at least partly distinct from that triggered by TCR/CD3.
研究領(lǐng)域 Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis

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使用細(xì)節(jié)

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應(yīng)用備注 Working Dilution:
FACS: 1 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
說明

In vitro T-cell costimulation

樣本量 1 mL
限制 For Research Use only

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貯存及處理

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緩沖液 Product is supplied as 0.5 mg in 1.0 mL of phosphate buffered saline, pH 7.4, containing 0.1 % sodium azide as preservative (PBS / NaN3).
儲存液 Sodium azide
注意事項(xiàng) WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediay. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事項(xiàng) Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.
儲存條件 4 °C

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