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CD8A抗體

抗原
CD8a Molecule (CD8A)
別名 cd8, LOC100136450, BB154331, Ly-2, Ly-35, Ly-B, Lyt-2, zgc:136643, CD8, Leu2, MAL, p32, RHACD8-4
適用
其他選擇 Chicken
Human(499),Murine (341),Rat ((Rattus)) (91),Dog (Canine) (51),Chicken (45),Monkey (25),Cat (24),Pig (Porcine) (18),Sheep (Ovine) (12),Bovine (10),Guinea Pig (8),Ferret (6),Rabbit (6),Turkey (5),Goat (4),Caprinae (2),Zebrafish (2),Mammalian (1)
宿主
其他選擇 Mouse
Mouse(616),Rat (308),Rabbit (137),Human (1)
克隆類型 (克隆位點(diǎn))
單克隆 (EP72)
標(biāo)記
其他選擇 FITC
FITC(166),PE (127),Biotin (105),APC (43),Cy5 (17),PerCP (17),PE-Cy5 (11),SPRD (8),PE-Cy7 (7),Alexa Fluor 488 (6),APC-Cy7 (5),HRP (5),APC eFluor 780 (4),Alexa Fluor 647 (4),Allophycocyanin (4),EFluor 450 (4),PE,Cy5 (4),Alexa Fluor 350 (3),Alexa Fluor 555 (3),Alexa Fluor 700 (3),Cy3 (3),Cy5,PE (3),Cy5.5 (3),Cy7 (3),DyLight 488 (3),DyLight 550 (3),DyLight 594 (3),DyLight 650 (3),DyLight 680 (3),DyLight 755 (3),PE-Cy5.5 (3),PE-TR (3),PerCP,Cy5.5 (3),EFluor 605NC (2),PE,Cy7 (2),PE-DyLight 594 (2),PE-Dyomics 590 (2),APC-Cy5.5 (1),CF405M (1),Cy5.5,PE (1),Cy7,PE (1),Dyomics 647 (1),EFluor 625NC (1),Magnetic Particles (1),PE-Dyomics 647 (1),Quantum Red (1),Sepharose (1),Texas Red (TR) (1)
應(yīng)用范圍
其他選擇 Flow Cytometry (FACS), Immunohistochemistry (IHC)
Flow Cytometry (FACS)(795),Immunohistochemistry (Frozen Sections) (IHC (fro)) (181),Immunohistochemistry (IHC) (164),Immunoprecipitation (IP) (144),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (101),Western Blotting (WB) (99),ELISA (73),Immunocytochemistry (ICC) (58),Immunofluorescence (IF) (48),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (30),Functional Studies (Func) (29),Immunohistochemistry (Acetone-fixed) (IHC (af)) (26),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (24),Cytotoxicity Test (CyTox) (15),Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (6),Blocking Reagent (BR) (5),Immunostaining (IS) (4),Enzyme Immunoassay (EIA) (3),Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3),Immunoassay (IA) (2),Blocking Antibody (Inhibition) (1),Fluorescence Microscopy (FM) (1),Microcytotoxicity Assay (MCA) (1),Radioimmunoassay (RIA) (1)
[Pubmed] 2 references available
規(guī)格 0.5 mg
發(fā)貨至 中國 (更改)
發(fā)貨時間 23至28個工作日

CD8A抗體

產(chǎn)品細(xì)節(jié)

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產(chǎn)品細(xì)節(jié)

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克隆位點(diǎn) EP72
亞型 IgG2b
產(chǎn)品特性 The antibody detects: chain of chicken CD8 (Mr 34 kDa)
MAb EP72 recognizes the CD8 chain.
純化方法 Protein A

目標(biāo)詳細(xì)情況

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目標(biāo)詳細(xì)情況

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別名 CD8a
背景 In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two chains, and (ii) a heterodimer of an chain and a chain. Chicken CD8 is expressed on approximay 80 % of thymocytes, 45 % of blood mononuclear cells and 50 % of spleen cells, but < 1 % of cells in the bursa and bone marrow. While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8- and CD8-chains, a relatively large proportion of the CD8+ TCR- cells in the spleens of embryos and young chicks express only the -chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR- CD8 cells in the chicken that is not found in rodents.
研究領(lǐng)域 CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)

使用細(xì)節(jié)

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使用細(xì)節(jié)

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應(yīng)用備注 Working Dilution:
FACS: 1 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
樣本量 1 mL
限制 For Research Use only

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貯存及處理

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緩沖液 Product is supplied as 0.5 mg in 1.0 mL of PBS / NaN3.
儲存液 Sodium azide
注意事項(xiàng) WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediay. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事項(xiàng) Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.
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