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STAT3抗體

參   考   價: 1800

訂  貨  量: ≥1 

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更新時間:2018-01-23 06:34:13瀏覽次數:787次

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STAT3抗體的概念:
Z初有人用電泳證明血清中抗體活性在γ球蛋白部分,故曾把抗體統(tǒng)稱為丙種(γ)球蛋白。后來發(fā)現,抗體并不都在γ區(qū);并且位于γ區(qū)的球蛋白,也不一定都具有抗體活性,規(guī)格:0.1ml/100µg、0.2ml/200µg

STAT3抗體

抗原
Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3)
別名 1110034C02Rik, AW109958, Aprf, APRF, HIES, Xstat3, aprf, hies, stat3, wu:fc15d02, wu:fl59g06, z-Stat3
抗原表位
其他選擇 N-Term
pTyr705(75),pSer727 (63),C-Term (47),Ser727 (22),N-Term (12),Tyr705 (12),Internal Region (10),AA 50-240 (9),AA 673-699 (9),AA 711-743 (8),pTyr708 (7),AA 725-729 (5),AA 1-175 (3),AA 335-546 (3),AA 4-31 (3),AA 703-707 (3),AA 154-175 (2),AA 563-670 (2),AA 683-709 (2),Middle Region (2),Tyr708 (2),AA 1-170 (1),AA 1-350 (1),AA 1-769 (1),AA 1-770 (1),AA 135-279 (1),AA 346-552 (1),AA 51-278 (1),AA 529-747 (1),AA 576-678 (1),AA 656-705 (1),AA 656-768 (1),AA 670-769 (1),AA 670-770 (1),AA 672-721 (1),AA 675-725 (1),AA 688-722 (1),AA 701-710 (1),AA 703-714 (1),AA 708-770 (1),AA 720-732 (1),AA 720-770 (1),AA 722-732 (1),Asp733 (1),Isoform 1 (1),Pro699 (1),Ser691 (1),Ser705 (1),Ser721 (1),Thr721 (1),Transcript Variant 3 (1),Tyr1222 (1),Tyr1229 (1),Tyr694 (1),non-phosphorylated (1),pThr3 (1),pTyr68 (1),pTyr727 (1),phosphorylated (1),phosphospecific (1)
適用
其他選擇 Murine, Rat ((Rattus)), Xenopus laevis, Pig (Porcine), Bovine, Dog (Canine), Zebrafish (Danio rerio), Human, Chicken
Human(386),Murine (194),Rat ((Rattus)) (162),Dog (Canine) (46),Bovine (44),Pig (Porcine) (39),Chicken (38),Guinea Pig (36),Zebrafish (15),Monkey (8),Rat (Meriones) (2),Hamster (1),Xenopus laevis (1),Zebrafish (Danio rerio) (1)
宿主
其他選擇 Rabbit
Rabbit(367),Mouse (54),Goat (9),Sheep (3),Chicken (1)
克隆類型
多克隆
標記
其他選擇 非結合性
Biotin(16),FITC (11),HRP (8),PE (6),APC (5),Alkaline Phosphatase (AP) (5),Alexa Fluor 647 (4),Alexa Fluor 350 (3),Alexa Fluor 488 (3),Alexa Fluor 555 (3),Cy3 (3),Cy5 (3),Cy5.5 (3),Cy7 (3),CF (1),Magnetic Particles (1),Sepharose (1)
應用范圍
其他選擇 Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)
Western Blotting (WB)(375),Immunohistochemistry (IHC) (164),ELISA (155),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (88),Immunoprecipitation (IP) (71),Immunofluorescence (IF) (60),Immunocytochemistry (ICC) (49),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (30),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (22),Immunohistochemistry (Frozen Sections) (IHC (fro)) (18),Enzyme Immunoassay (EIA) (11),Flow Cytometry (FACS) (11),Dot Blot (DB) (5),Gel Shift (GS) (3),Proximity Ligation Assay (PLA) (3),Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2),Immunoassay (IA) (1)
[Pubmed] 1 reference available
規(guī)格 50 μg
發(fā)貨至 中國 (更改)
發(fā)貨時間 9至12個工作日

STAT3抗體

產品細節(jié)

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產品細節(jié)

顯示 隱藏
免疫原 Synthetic peptide directed towards the N terminal of human STAT3
序列 AQWNQLLD TRYLEQLHQL YSDSFPMELR QFLAPWIESQ DWAYAASKES
預測反應 Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, Zebrafish : 70 %
產品特性 This is a rabbit polyclonal antibody against STAT3. It was validated on Western Blot using a cell lysate as a positive control.
純化方法 Affinity Purified

目標詳細情況

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目標詳細情況

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別名 STAT3
背景 STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. It mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein.The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described.
分子量 88 kDa
基因ID 6774
NCBI Accession NP_003141, NM_003150
UniProt B5BTZ6
研究領域 Embryogenesis, Embryonic stem cells, Transcription Factors, Signaling, Receptors, Inflammation

使用細節(jié)

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使用細節(jié)

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應用備注 Optimal working dilutions should be determined experimentally by the investigator.
說明

Antigen size: 769 AA

限制 For Research Use only

貯存及處理

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貯存及處理

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狀態(tài) Lyophilized
溶解方式 Add 50 µL of distilled water.
濃度 1 mg/mL
緩沖液 PBS buffer with 2 % sucrose
注意事項 Avoid repeated freeze-thaw cycles.
儲存條件 -20 °C
儲存方法 For longer periods of storage, store at -20 °C

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