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IL12B抗體

抗原
Interleukin 12b (IL12B)
別名 IL-12B, IL12B, p40, Il-12b, Il12p40, Il-12p40, Il12, CLMF, CLMF2, NKSF, NKSF2, IL12p40, IL-12, IL-12.p40, IL-12p40
抗原表位
其他選擇 AA 1-328
Internal Region(14),AA 23-328 (11),AA 1-328 (7),AA 271-298 (5),C-Term (5),AA 195-222 (4),AA 229-328 (2),AA 228-277 (1),Val277 (1)
適用
其他選擇 Human
Human(81),Murine (21),Sheep (Ovine) (13),Rat ((Rattus)) (6),Bovine (3),Fish (1)
宿主
其他選擇 Rabbit
Rabbit(50),Mouse (37),Goat (11),Rat (10),Sheep (4),Chicken (1),Human (1)
克隆類(lèi)型
多克隆
標(biāo)記
其他選擇 非結(jié)合性
Biotin(5),FITC (5),HRP (4),PE (4),APC (2),Alkaline Phosphatase (AP) (2)
zui低檢測(cè)濃度 0.00245 ng/well
應(yīng)用范圍
其他選擇 ELISA
Western Blotting (WB)(84),ELISA (82),Immunohistochemistry (IHC) (20),Flow Cytometry (FACS) (16),Enzyme Immunoassay (EIA) (8),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7),Immunohistochemistry (Frozen Sections) (IHC (fro)) (4),Immunoprecipitation (IP) (4),Functional Studies (Func) (3),Dot Blot (DB) (2),Immunoassay (IA) (2),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (2),Neutralization (Neut) (2),ELISpot (1),Immunocytochemistry (ICC) (1),Immunofluorescence (IF) (1),Radioimmunoassay (RIA) (1)
[Pubmed] 2 references available
規(guī)格 100 μg
發(fā)貨至 中國(guó) (更改)

IL12B抗體

產(chǎn)品細(xì)節(jié)

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產(chǎn)品細(xì)節(jié)

顯示 隱藏
亞型 IgG
特異性 Human IL12B / P40 / NKSF2
產(chǎn)品特性 Produced in rabbits immunized with purified, recombinant Human IL12B (rhIL12B, Met 1-Ser 328, NP_002178.2). Total IgG was purified by Protein A affinity chromatography.
純化方法 Affinity Purified
過(guò)濾 0.2 μm filtered

目標(biāo)詳細(xì)情況

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目標(biāo)詳細(xì)情況

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別名 IL12B
背景 Subunit beta of interleukin 12 (also known as natural killer cell stimulatory factor 2, or cytotoxic lymphocyte maturation factor 2, p40) (IL12B) is a subunit of human interleukin 12. IL12B/IL-12B is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. IL12B/IL-12B is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. IL12B/IL-12B associates with IL23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity.
NCBI Accession NP_002178.2
研究領(lǐng)域 Hormones, Cytokines

使用細(xì)節(jié)

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使用細(xì)節(jié)

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應(yīng)用備注 ELISA ELISA: 0.5-1.0 μg/mL This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human IL12B. The detection limit for Human IL12B is 0.00245 ng/well.
限制 For Research Use only

貯存及處理

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貯存及處理

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緩沖液 PBS with 5% trehalose
儲(chǔ)存液 Without preservative
注意事項(xiàng) Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
儲(chǔ)存條件 4 °C/-20 °C/-80 °C
儲(chǔ)存方法 This antibody can be stored at 2 °C-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C.
有效期 12 months

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