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Drosophila抗體 

抗原
Notum Pectinacetylesterase Homolog (Drosophila) (NOTUM)
別名 5730593N15Rik, RGD1307119
抗原表位
其他選擇 N-Term
C-Term(8),AA 352-379 (7),N-Term (7),AA 353-379 (2)
適用
其他選擇 Human, Murine, Rat ((Rattus))
Human(35),Murine (21),Rat ((Rattus)) (19),Bovine (12),Dog (Canine) (12)
宿主
其他選擇 Rabbit
Rabbit(34),Mouse (1)
克隆類型
多克隆
標(biāo)記
其他選擇 非結(jié)合性
Biotin(2),FITC (2),HRP (2),APC (1),Alexa Fluor 350 (1),Alexa Fluor 488 (1),Alexa Fluor 555 (1),Alexa Fluor 647 (1),Alkaline Phosphatase (AP) (1),Cy3 (1),Cy5 (1),Cy5.5 (1),Cy7 (1),PE (1)
應(yīng)用范圍
其他選擇 Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB)(26),ELISA (15),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (10),Immunohistochemistry (IHC) (10),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3),Immunohistochemistry (Frozen Sections) (IHC (fro)) (2),Immunoassay (IA) (1),Immunocytochemistry (ICC) (1),Immunofluorescence (IF) (1)
[Pubmed] 1 reference available
規(guī)格 0.1 mg
發(fā)貨至 中國(guó) (更改)
發(fā)貨時(shí)間 29至35個(gè)工作日

Drosophila抗體 

產(chǎn)品細(xì)節(jié)

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產(chǎn)品細(xì)節(jié)

顯示 隱藏
免疫原 NOTUM antibody was raised against a 19 amino acid peptide near the amino terminus of Human NOTUM.
亞型 IgG
特異性 This antibody detects Notum.
交叉反應(yīng) （詳細(xì)） Species reactivity (tested):Human, Mouse, Rat.
純化方法 Peptide Affinity Chromatography

 目標(biāo)詳細(xì)情況

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目標(biāo)詳細(xì)情況

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別名 Notum
背景 In Drosophila, the notum gene is regulated by the Wingless pathway and encodes a secreted hydrolase that modifies heparan sulfate proteoglycans. The mammalian homolog has been shown to be able to cleave glypicans and can release GPI-anchored proteins from the mammalian cell surface. Like the Drosophilia NOTUM, the mammalian protein can act as a negative regulator of the Wnt signaling pathway. NOTUM is expressed at a low level in most mammalian tissues, although it is overexpressed in a subset of human hepatocellular carcinomas. Its transcription is regulated by b-catenin/TCF and is a target of the Wnt signaling pathway, forming a negative feedback loop that regulates the expression and activity of the Wnt pathway.
基因ID 147111
NCBI Accession NP_848588
UniProt Q6P988

 使用細(xì)節(jié)

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使用細(xì)節(jié)

顯示 隱藏
應(yīng)用備注 ELISA. Western blot: 0.5 - 1?μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 For Research Use only

貯存及處理

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貯存及處理

顯示 隱藏
濃度 1.0 mg/mL
緩沖液 PBS containing 0.02?% Sodium Azide
儲(chǔ)存液 Sodium azide
注意事項(xiàng) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事項(xiàng) Avoid repeated freezing and thawing.
儲(chǔ)存條件 4 °C/-20 °C

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