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染色體結(jié)構(gòu)維持蛋白1抗體

英文名稱(chēng) SMC1
中文名稱(chēng) 染色體結(jié)構(gòu)維持蛋白1抗體
別    名 CDLS2; SMC1; Segregation of mitotic chromosomes 1; Segregation of mitotic chromosomes like 1; SMC 1; SMC1A; SMC1alpha; SMC1alpha protein; DXS423E; KIAA0178; SB1.8; SMC1B; SMC1BETA; SMC1beta protein; SMC1L1; SMC1L2; SMCB; Structural maintenance of chromosome 1 like 1 protein; Structural maintenance of chromosome 1 like 2 protein; structural maintenance of chromosomes 1-like 2 (yeast); Structural maintenance of chromosomes 1A; Structural maintenance of chromosomes 1B; Structural Maintenance of Chromosomes-1 Like 1; SMC1B_HUMAN.

說(shuō) 明 書(shū) 0.2ml  
研究領(lǐng)域 腫瘤  免疫學(xué)  染色質(zhì)和核信號(hào)  轉(zhuǎn)錄調(diào)節(jié)因子  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, 
產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 136kDa
細(xì)胞定位 細(xì)胞核 
性    狀 Lyophilized or Liquid
濃    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human SMC1(147-197aa)
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
產(chǎn)品介紹 background:
Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Function:
Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesion complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

Subunit:
Interacts with POLE. Interacts with SYCP2. Interacts with BRCA1. Found in a complex with CDCA5, SMC3 and RAD21, PDS5A/APRIN and PDS5B/SCC-112 (By similarity). Forms a heterodimer with SMC3 in cohesin complexes. Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. In germ cell cohesion complexes, SMC1A is mutually exclusive with SMC1B. Interacts with BRCA1. Interacts with NDC80.

Subcellular Location:
Nucleus. Chromosome. Chromosome, centromere, kinetochore. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesion complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesion complexes remain. At anaphase, the RAD21 subunit of the cohesion complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesion complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere-kinetochore complex at the kinetochore region during mitosis.

Post-translational modifications:
Phosphorylated by ATM upon ionizing radiation in a NBS1-dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation.

DISEASE:
Defects in SMC1A are the cause of Cornelia de Lange syndrome type 2 (CDLS2) [MIM:300590]; also known as Cornelia de Lange syndrome X-linked. CDLS is a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. CDLS is characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation and various other malformations including gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies.

Similarity:
Belongs to the SMC family. SMC1 subfamily.xy-6782R    MST4絲氨酸/蘇氨酸蛋白激酶MST4抗體
xy-7761R    Myosin VIIa肌球蛋白7a/常染色體隱性耳聾蛋白2抗體
xy-8084R    MBC1膀胱癌突變蛋白1抗體
xy-9376R    MURF3肌肉特定環(huán)指蛋白3抗體
xy-9377R    EDPF1腸細(xì)胞分化相關(guān)因子1抗體
xy-9379R    RNF62環(huán)指蛋白62抗體
xy-6933R    MICBMHC I類(lèi)鏈相關(guān)蛋白B
xy-7115R    MLC1膜蛋白MLC1抗體
xy-3278R    Phospho-MKP1 (Ser359)磷酸化絲裂原活化蛋白激酶磷酸酶-1抗體
xy-3279R    Phospho-MKP1 (Ser296)磷酸化絲裂原活化蛋白激酶磷酸酶-1抗體
xy-2992R    MLK3絲氨酸/蘇氨酸蛋白激酶MLK3抗體
xy-3280R    Phospho-MLK3(Thr277 + Ser281)磷酸化絲氨酸/蘇氨酸蛋白激酶MLK3抗體
xy-2995R    MSK1有絲分裂原和應(yīng)激活化型蛋白激酶1抗體
xy-3281R    Phospho-MSK1 (Ser360)磷酸化有絲分裂原和應(yīng)激活化型蛋白激酶1抗體
xy-3282R    Phospho-MSK1 (Ser212)磷酸化有絲分裂原和應(yīng)激活化型蛋白激酶1抗體
xy-3283R    Phospho-MSK1 (Ser376)磷酸化有絲分裂原和應(yīng)激活化型蛋白激酶1抗體
xy-3284R    Phospho-MSK1 (Thr581)磷酸化有絲分裂原和應(yīng)激活化型蛋白激酶1抗體
xy-2998R    MYPT1肌球蛋白磷酸酶抗體
xy-3285R    Phospho-MYPT1 (Ser507)磷酸化肌球蛋白磷酸酶抗體
xy-3286R    Phospho-MYPT1 (Ser668)磷酸化肌球蛋白磷酸酶抗體
xy-3287R    Phospho-MYPT1(Thr696)磷酸化肌球蛋白磷酸酶抗體
xy-3288R    Phospho-MYPT1 (Thr853)磷酸化肌球蛋白磷酸酶抗體
xy-3273R    Phospho-Met (Tyr1349)磷酸化肝細(xì)胞生長(zhǎng)因子受體(原癌基因)抗體
xy-2911R    MEK6絲裂原活化蛋白激酶MKK6抗體
xy-3274R    Phospho-MKK3(Ser218) + MKK6(Ser207) 磷酸化絲裂原活化蛋白激酶MKK3/6抗體
xy-3275R    Phospho-MEK6 (Ser207)磷酸化絲裂原活化蛋白激酶MKK6抗體
xy-1761R    MDC嗜酸粒細(xì)胞趨化蛋白22抗體
xy-1759R    Met Enkephalin甲硫氨酸腦啡肽抗體
xy-2129R    Morphine嗎啡抗體
xy-2099R    MMP-9基質(zhì)金屬蛋白酶9抗體
xy-1882R    MRas原癌基因M-ras抗體
xy-1955R    MCP1單核細(xì)胞趨化蛋白1抗體
xy-2029R    Morphine嗎啡抗體