細胞周期后期促進蛋白亞基10抗體 返回列表頁

英文名稱 APC10
中文名稱 細胞周期后期促進蛋白亞基10抗體
別    名 ANAPC 10; anapc10; Anaphase promoting complex 10; Anaphase promoting complex subunit 10; Anaphase-promoting complex subunit 10; Apc 10; APC10; APC10_HUMAN; Cyclosome subunit 10; DKFZP564L0562; Doc 1; Doc1; OTTHUMP00000220297; OTTHUMP00000220298; OTTHUMP00000220299; OTTHUMP00000220300; OTTHUMP00000220303.
細胞周期后期促進蛋白亞基10抗體    
說 明 書 0.1ml  0.2ml  
研究領(lǐng)域 細胞生物  細胞周期蛋白  泛素  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Pig, Cow, Rabbit, Sheep, 
產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 21kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human APC10
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
Composed of more than ten subunits, the anaphase-promoting complex (APC) acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis. APC, or cyclosome, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division. APC is phosphorylated, and thus activated, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk). APC is under tight control by a number of regulatory factors, including CDC20, CDH1 and MAD2. Specifically, CDC20 and CDH1 directly bind to and activate the cyclin-ubiquitination activity of APCs. In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC, thus preventing APC activation. APC10 contains a Doc1 homology domain, which is a beta-sandwich structure common to many other putative E3 ubiquitin ligases. APC10 binds to core APC subunits throughout the cell cycle. Specifically, APC10 binds to the C-terminus of CDC27/APC3. During mitosis, APC10 is localized in centrosomes and mitotic spindles. APC10 also localizes to kinetochores from prophase to anaphase, and to the midbody in ophase and cytokinesis.

Function:
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Subunit:
The APC/C is composed of at least 12 subunits. The C-terminus of APC10 binds to CDC27/APC3.

Similarity:
Belongs to the APC10 family.
Contains 1 DOC domain.

Database links:
UniProtKB/Swiss-Prot: Q9UM13.1

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.xy-5510R Phospho-NFKB1 (Thr931)磷酸化細胞核因子p50/k基因結(jié)合核因子抗體
xy-5511R Phospho-NFKB1 (Ser907)磷酸化細胞核因子p50/k基因結(jié)合核因子抗體
xy-5512R Phospho-NFKB1 (Ser337)磷酸化細胞核因子p50/k基因結(jié)合核因子抗體
xy-5513R Phospho-NFKB1 (Ser893)磷酸化細胞核因子p50/k基因結(jié)合核因子抗體
xy-5514R Phospho-NFKBIA (Tyr42) 磷酸化IKB α抗體
xy-5515R phospho-NFKBIA(Tyr305)磷酸化IKB alpha抗體
xy-5516R phospho-NFATc2(Ser330)磷酸化核因子活化T細胞胞漿蛋白2抗體
xy-10156R NOX5還原型輔酶Ⅱ氧化酶5/煙酰胺腺嘌呤二核苷酸磷酸抗體
xy-6314R NANOS2生殖細胞增殖相關(guān)蛋白NANOS2抗體
xy-3622R NFKB p52細胞核因子/k基因結(jié)合核因子 p52/p100抗體
xy-3723R Phospho-NFKB2(Ser866+Ser870)磷酸化細胞核因子/k基因結(jié)合核因子p100抗體
xy-2798R NAT2N-乙?；D(zhuǎn)移酶2抗體
xy-6717R NALP2富含亮氨酸重復(fù)結(jié)構(gòu)域蛋白2抗體
xy-4636R NR2C2孤兒核受體TAK1抗體
xy-10227R Nanos3骨巢蛋白抗體
xy-13710R Netrin 5軸突生長誘向因子5抗體
xy-2513R phospho-NFKBIA(Ser32/36)磷酸化p-IκB-α抗體
xy-6079R G21 proteinG21蛋白質(zhì)抗體
xy-2184R NQO1醌氧化還原酶抗體
xy-1070R NPY1R神經(jīng)肽Y1受體抗體
xy-1068R NMDAR1 谷氨酸受體1抗體
xy-5968R NARG2谷氨酸受體調(diào)節(jié)蛋白2抗體
xy-6077R NIT1腈水解酶1抗體
xy-6349R Nerve growth factor inducible神經(jīng)生長因子誘導(dǎo)蛋白抗體
xy-7665R NUP50核孔蛋白50抗體