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當(dāng)前位置:上海信裕生物科技有限公司>>抗體>>一抗>> 溴脫氧尿苷單克隆抗體(增殖標(biāo)志物)
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更新時間:2017-12-13 20:05:21瀏覽次數(shù):457次
聯(lián)系我時,請告知來自 化工儀器網(wǎng)英文名稱 BrdU (A7)
中文名稱 溴脫氧尿苷單克隆抗體(增殖標(biāo)志物)
別 名 Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker.
溴脫氧尿苷單克隆抗體(增殖標(biāo)志物)
說 明 書 0.1ml 0.2ml
產(chǎn)品類型 藥物與化合物抗體
研究領(lǐng)域 細(xì)胞生物 免疫學(xué) 發(fā)育生物學(xué) 染色質(zhì)和核信號 神經(jīng)生物學(xué) 藥物及化合物 細(xì)胞類型標(biāo)志物
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號 A7
交叉反應(yīng) BrdU
產(chǎn)品應(yīng)用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 0.3071kDa
細(xì)胞定位 細(xì)胞核
性 狀 Lyophilized or Liquid
濃 度 1mg/1ml
免 疫 原 bromodeoxyuridine conjugated to KLH
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 Preservative: 15mM Sodium Azide, Constituents: 0.01M PBS, pH 7.4
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
Proliferation Marker
Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. Anti-bromodeoxyuridine monoclonal antibody has been used for identi-fying proliferating cells in blood (Campana et al., 1988), tissues (Schutte et al., 1987; Hayashi, et al., 1988), tumors (Hoshino et al., 1986; Morstyn et al., 1983), as well as for determining plasma cell labeling indices (Greipp et al., 1985).
Subcellular Location:
Nuclear.
Important Note:
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