原癌蛋白CBL2抗體 返回列表頁

英文名稱 CBL2
中文名稱 原癌蛋白CBL2抗體
別    名 C CBL; Cas-Br-M (murine) ecotropic retroviral transforming sequence; Casitas B lineage lymphoma proto oncogene; CBL 2;E3 ubiquitin protein ligase CBL; Oncogene CBL2; Proto oncogene c CBL; RGD1561386; RING finger protein 55; RNF55v Signal transduction protein CBL; 4732447J05Rik; CBL_HUMAN.
原癌蛋白CBL2抗體  
說 明 書 0.2ml  
研究領域 腫瘤  細胞生物  染色質和核信號  神經(jīng)生物學  信號轉導  細胞凋亡  生長因子和激素  轉錄調(diào)節(jié)因子  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, 
產(chǎn)品應用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 100kDa
細胞定位 細胞漿 
性    狀 Lyophilized or Liquid
濃    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human CBL2
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia. Mutations in this gene are also the cause of Noonan syndrome-like disorder. [provided by RefSeq, Mar 2012]

Function:
Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, EGFR, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The Tyr-731 phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function.

Subunit:
Interacts (phosphorylated at Tyr-731) with PIK3R1. Associates with NCK via its SH3 domain. The phosphorylated C-terminus interacts with CD2AP via its second SH3 domain. Binds to UBE2L3. Interacts with adapters SLA, SLA2 and with the phosphorylated C-terminus of SH2B2. Interacts with EGFR, SYK and ZAP70 via the highly conserved Cbl-N region. Also interacts with SORBS1 and INPPL1/SHIP2. Interacts with phosphorylated LAT2. May interact with CBLB (By similarity). Interacts with ALK, AXL, BLK, FGR and FGFR2. Interacts with CSF1R, EPHB1, FLT1, KDR, PDGFRA and PDGFRB; regulates receptor degradation through ubiquitination. Interacts with HCK and LYN. Interacts with TEK/TIE2 (tyrosine phosphorylated).

Subcellular Location:
Cytoplasm. Cell membrane. Note=Colocalizes with FGFR2 in lipid rafts at the cell membrane.

Post-translational modifications:
Phosphorylated on tyrosine residues by ALK, EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues in response to FLT1 and KIT signaling. Phosphorylated on tyrosine residues by INSR and FGR. Phosphorylated on several tyrosine residues by constitutively activated FGFR3. Not phosphorylated at Tyr-731 by FGFR3. Phosphorylated on tyrosine residues by activated CSF1R, PDGFRA and PDGFRB. Phosphorylated on tyrosine residues by HCK.
Ubiquitinated, leading to its degradation via the proteasome.

DISEASE:
Noonan syndrome-like disorder with or without juvenile myelomonocytic leukemia (NSLL) [MIM:613563]: A syndrome characterized by a phenotype reminiscent of Noonan syndrome. Clinical features are highly variable, including facial dysmorphism, short neck, developmental delay, hyperextensible joints and thorax abnormalities with widely spaced nipples. The facial features consist of triangular face with hyperorism, large low-set ears, ptosis, and flat nasal bridge. Some patients manifest cardiac defects. Some have an increased risk for certain malignancies, particularly juvenile myelomonocytic leukemia. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.
Contains 1 RING-type zinc finger.
Contains 1 UBA domain.

Gene ID:
867

Database links:

Entrez Gene: 867 Human

Entrez Gene: 12402 Mouse

Entrez Gene: 500985 Rat

Omim: 165360 Human

SwissProt: P22681 Human

SwissProt: P22682 Mouse

Unigene: 504096 Human

Unigene: 266871 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.xy-5743R CDKN3細胞周期蛋白依賴性激酶抑制蛋白3抗體
xy-11187R CK42細胞角蛋白42
xy-4609R CD105CD105抗體
xy-2322R Caspase-7半胱胺酸蛋白酶蛋白-7抗體
xy-4602R CD8CD8/CD8-β抗體
xy-5744R CABLES2細胞周期蛋白依賴性激酶CABLES2抗體
xy-0432R Chicken IgY兔抗雞IgY抗體
xy-15122R C20orf12820號染色體開放閱讀框128抗體
xy-4562R CYP2E1細胞色素P450ⅡE1抗體
xy-4509R CXCL13/BCA1B-淋巴細胞趨化因子抗體
xy-5740R Cdc23細胞分裂周期蛋白23抗體
xy-5738R CKAP5結腸和肝腫瘤高表達蛋白抗體
xy-5745R CKS1細胞周期蛋白依賴性激酶調(diào)節(jié)亞基1抗體
xy-4614R ICAM1細胞間粘附分子-1抗體
xy-4615R ICAM1細胞間粘附分子1抗體
xy-4616R ICAM1細胞間粘附分子-1抗體
xy-4617R ICAM1細胞間粘附分子-1抗體
xy-4618R ICAM1細胞間粘附分子-1抗體
xy-7624R Cyclophilin F親環(huán)蛋白（親環(huán)素）PPIF抗體
xy-6874R Catalase過氧化氫酶抗體
xy-7098R CLIC4細胞內(nèi)氯離子通道蛋白4抗體
xy-6373R CGR19細胞生長調(diào)控蛋白19/環(huán)指蛋白197抗體
xy-7102R CCDC139卷曲螺旋結構域蛋白139抗體
xy-6375R CHMP1A染色體修飾蛋白1抗體（金屬蛋白酶1）
xy-11812R CAPS1鈣依賴分泌激活蛋白1抗體
xy-11814R CBLN1小腦肽1抗體