CD90 （THY1） 抗體 （PE） 返回列表頁

CD90 (THY1) 抗體 (PE)

抗原
CD90 (THY1)
別名 CD90, CD7, T25, Thy-1, Thy-1.2, Thy1.1, Thy1.2
適用
其他選擇 Murine
Murine(309),Human (144),Rat (Rattus) (121),Dog (Canine) (26),Pig (Porcine) (22),Guinea Pig (14),Rabbit (14),Monkey (11),Zebrafish (Brachydanio rerio) (5),Non-Human Primate (4),Bovine (2),Horse (Equine) (1)
宿主
其他選擇 Mouse
Rat(220),Mouse (203),Rabbit (76),Goat (5)
克隆類型 (克隆位點)
Monoclonal (5a-8)
標記
其他選擇 PE
Biotin(65),FITC (64),PE (54),APC (23),Cy5,PE (7),PerCP (4),SPRD (4),Cy7,PE (3),DyLight 488 (3),DyLight 550 (3),DyLight 594 (3),DyLight 650 (3),DyLight 680 (3),DyLight 755 (3),APC eFluor 780 (2),EFluor 450 (2),EFluor 605NC (2),HRP (2),PE-Cy5 (2),PE-Cy7 (2),Alexa Fluor 488 (1),Alexa Fluor 555 (1),Alexa Fluor 647 (1),Alkaline Phosphatase (AP) (1),Allophycocyanin (1),Cy3 (1),Cy5 (1),Cy5.5 (1),Cy7 (1),PE,Cy5 (1),PE,Cy7 (1),PE-Cy3 (1),PE-Cy5.5 (1),PerCP,Cy5.5 (1)
應用范圍
其他選擇 Flow Cytometry (FACS)
Flow Cytometry (FACS)(370),Western Blotting (WB) (105),Immunohistochemistry (Frozen Sections) (IHC (fro)) (98),Immunoprecipitation (IP) (61),ELISA (50),Immunohistochemistry (IHC) (46),Immunocytochemistry (ICC) (35),Cytotoxicity Test (CyTox) (32),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (25),Immunofluorescence (IF) (19),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (9),Functional Studies (Func) (7),Immunoassay (IA) (2),Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2),Immunostaining (IS) (2),Cell Culture (CC) (1),Immunomicroscopy (IM) (1),Purification (Purif) (1)
[Pubmed] 4 references available
規(guī)格 50 μg
發(fā)貨至 中國 (Change)
CD90 (THY1) 抗體 (PE)

目標詳細情況

顯示 隱藏
別名 CD90
背景 CD90 (Thy1) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.
Alternate names: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein
基因ID 21838
NCBI Accession NP_033408
UniProt P01831
研究領域 CD Antigens, Surface Receptors of Immune Cells, Experimental Models

使用細節(jié)

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使用細節(jié)

顯示 隱藏
應用備注 Flow Cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
實驗流程 FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 5 µg* of this Ab. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 5 µg/10e6 cellsIsotypic Control: PE-Mouse IgG2b,kCell Source Percentage of cells stained above control: Thymus: 98. 4%Spleen: 28. 8%Results - Strain Distribution: Cell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 5 µg/10e6 cellsStrains Tested: C57BL/6, C3H/He, CBA/J, BALB/c, ATL, AKR/JPositive: C57BL/6, C3H/He, CBA/J, BALB/c, ATLNegative: AKR/J
限制 For Research Use only

貯存及處理

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貯存及處理

CD90 (THY1) 抗體 (PE)顯示 隱藏
狀態(tài) Liquid
濃度 0.1 mg/mL
緩沖液 PBS containing 0.02% Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
儲存液 Sodium azide
注意事項 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
CD90 (THY1) 抗體 (PE)注意事項 This product is photosensitive and should be protected from light
儲存條件 4 °C
儲存方法 Store the antibody undiluted at 2-8°C. DO NOT FREEZE!
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