Macrophage F4/80 Gen 抗體 返回列表頁

Macrophage F4/80 Gen 抗體

抗原
Macrophage F4/80 Gen
適用
其他選擇 Murine
Murine(29),Human (7),Rat (Rattus) (3)
宿主
其他選擇 Rat
Rat(20),Rabbit (9)
克隆類型 (克隆位點)
Monoclonal (CI:A3-1)
標記
其他選擇 PE
FITC(4),Biotin (3),PE (2),APC (1)
應用范圍
其他選擇 Flow Cytometry (FACS), Immunofluorescence (IF)
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))(24),Flow Cytometry (FACS) (19),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (18),Immunohistochemistry (Frozen Sections) (IHC (fro)) (15),Western Blotting (WB) (11),Immunoprecipitation (IP) (7),Radioimmunoassay (RIA) (6),Immunoelectron Microscopy (IEM) (3),Immunofluorescence (IF) (3),Functional Studies (Func) (1),Immunohistochemistry (IHC) (1),Immunohistochemistry (Resin Sections) (IHC (rs)) (1)
[Pubmed] 13 references available
規(guī)格 100 tests
發(fā)貨至 中國 (Change)
發(fā)貨時間 29至35個工作日

Macrophage F4/80 Gen 抗體

產(chǎn)品細節(jié)

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免疫原 Thioglycollate stimulated peritoneal cavity macrophages from C57/BL mice. Remarks: Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS-1 mouse myeloma cell line.
克隆位點 CI:A3-1
亞型 IgG2b
特異性 This antibody recognises the F4/80 antigen. Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes (5). We recommende the use of Cat.-No BM4008LE for this purpose.
交叉反應 （詳細） Species reactivity (tested):Mouse.
純化方法 Affinity Chromatography on Protein A

目標詳細情況

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目標詳細情況

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別名 Macrophage F4/80 Antigen
背景 Murine F4/80 antigen is a 160 kD cell surface glycoprotein that is a member of the EGF-TM7 family of proteins which shares 68?% overall amino acid identity with Human EGF module-containing mucin-like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.Synonyms: Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480
基因ID 13733
NCBI Accession NP_034260
UniProt Q61549

使用細節(jié)

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使用細節(jié)

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應用備注 Flow Cytometry: use 10 μL of neat antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 For Research Use only

貯存及處理

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貯存及處理

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溶解方式 Restore with 1?mL distilled water.
緩沖液 PBS,?pH 7.4, 0.09?% Sodium Azide, 1?% BSA, 5?% Sucrose
儲存液 Sodium azide
注意事項 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事項 This product is photosensitive and should be protected from light
儲存條件 4 °C
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