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CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) 抗體

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CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) 抗體的概念:
Z初有人用電泳證明血清中抗體活性在γ球蛋白部分,故曾把抗體統(tǒng)稱為丙種(γ)球蛋白。后來發(fā)現(xiàn),抗體并不都在γ區(qū);并且位于γ區(qū)的球蛋白,也不一定都具有抗體活性,規(guī)格:0.1ml/100µg、0.2ml/200µg

CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) 抗體

抗原
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E)
別名 cd3e, CD3E, t3e, tcre, AI504783, CD3, CD3epsilon, T3e, T3E, TCRE
適用
其他選擇 Murine
Murine(273),Human (166),Pig (Porcine) (36),Rat ((Rattus)) (31),Non-Human Primate (6),Cat (1),Dog (Canine) (1),Horse (Equine) (1),Monkey (1),Primate (1)
宿主
其他選擇 Hamster
Hamster(162),Mouse (114),Rabbit (100),Rat (48),Armenian Hamster (16),Syrian Hamster (11)
克隆類型 (克隆位點)
單克隆 (145-2C11)
標記
其他選擇 FITC
FITC(51),Biotin (44),PE (42),APC (24),Cy5 (10),SPRD (10),Cy5,PE (9),PerCP (7),Alexa Fluor 488 (5),HRP (5),Alexa Fluor 647 (4),Alexa Fluor 700 (4),PE,Cy5 (4),Alexa Fluor 350 (3),Alexa Fluor 555 (3),Allophycocyanin (3),Cy3 (3),Cy5.5 (3),Cy7 (3),Cy7,PE (3),APC eFluor 780 (2),DyLight 488 (2),DyLight 550 (2),DyLight 594 (2),DyLight 650 (2),DyLight 755 (2),EFluor 450 (2),PE,Cy7 (2),PerCP,Cy5.5 (2),Alkaline Phosphatase (AP) (1),Cy5.5,PE (1),DyLight 680 (1),Dyomics 647 (1),PE-Cy5 (1),PE-Dyomics 590 (1),PE-Dyomics 647 (1)
應用范圍
其他選擇 Flow Cytometry (FACS), Immunohistochemistry (IHC)
Flow Cytometry (FACS)(339),Immunoprecipitation (IP) (117),Western Blotting (WB) (100),Immunohistochemistry (IHC) (87),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (53),Immunohistochemistry (Frozen Sections) (IHC (fro)) (35),ELISA (32),Functional Studies (Func) (32),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (30),Immunocytochemistry (ICC) (20),Immunofluorescence (IF) (18),Blocking Reagent (BR) (3),Activation (Act) (1),Agonist (Agon) (1),Fluorescence Microscopy (FM) (1),Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (1)
[Pubmed] 3 references available
規(guī)格 0.1 mg
發(fā)貨至 中國 (更改)
發(fā)貨時間 23至28個工作日

CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) 抗體

產(chǎn)品細節(jié)

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免疫原 H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
克隆位點 145-2C11
亞型 IgG
產(chǎn)品特性 The antibody detects: Mouse CD3 (Mr 25 kDa)
The 145-2C11 monoclonal antibody recognizes an epitope on the 25 kDa chain of the CD3/TCR complex. In the presence of Fc receptor-bearing accessory cells, soluble 145-2C11 can activate primed and nave T cell in vitro. 145-2C11 can also induce redirected lysis of specific target cells by CTL clones and it can block lysis of specific target cells by antigen-specific CTL's. Immobilized 145-2C11 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by 145-2C11 may result in apoptotic cell death.

目標詳細情況

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CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) 抗體目標詳細情況

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別名 CD3e
背景 CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation.
研究領域 CD Antigens, Surface Receptors of Immune Cells

使用細節(jié)

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使用細節(jié)

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應用備注 Working Dilution:
FACS: 3 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
說明

In vitro depletion of CD3+ cells, In vitro activation of T cells

樣本量 1 mL
限制 For Research Use only

貯存及處理

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貯存及處理

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緩沖液 Product is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS / NaN3.
儲存液 Sodium azide
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) 抗體注意事項 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediay. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事項 Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.
儲存條件 4 °C

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