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Deiodinase, Iodothyronine, Type II (DIO2) (Interna

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Deiodinase, Iodothyronine, Type II (DIO2) (Internal Region) 抗體的概念:
Z初有人用電泳證明血清中抗體活性在γ球蛋白部分,故曾把抗體統(tǒng)稱為丙種(γ)球蛋白。后來發(fā)現(xiàn),抗體并不都在γ區(qū);并且位于γ區(qū)的球蛋白,也不一定都具有抗體活性,規(guī)格:0.1ml/100µg、0.2ml/200µg

Deiodinase, Iodothyronine, Type II (DIO2) (Internal Region) 抗體

抗原
Deiodinase, Iodothyronine, Type II (DIO2)
別名 Deio2, cb684, DIO2, D2, dio2-A, 5dii, sely, txdi2, 5DII, AI324267, DIOII, SelY, TXDI2
抗原表位
其他選擇 Internal Region
Internal Region(10),AA 165-191 (2),AA 171-200 (2),AA 30-259 (2),Center (1),Middle Region (1)
適用
其他選擇 Murine
Human(27),Murine (14),Rat ((Rattus)) (14),Pig (Porcine) (13),Bovine (12),Dog (Canine) (12)
宿主
其他選擇 Goat
Rabbit(25),Goat (4)
克隆類型
多克隆
標(biāo)記
其他選擇 非結(jié)合性
Biotin(2),FITC (2),HRP (2),APC (1),Alexa Fluor 350 (1),Alexa Fluor 488 (1),Alexa Fluor 555 (1),Alexa Fluor 647 (1),Alkaline Phosphatase (AP) (1),Cy3 (1),Cy5 (1),Cy5.5 (1),Cy7 (1),PE (1)
實(shí)驗(yàn)類型 Peptide ELISA
應(yīng)用范圍
其他選擇 ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Western Blotting (WB)(16),ELISA (11),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (10),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9),Flow Cytometry (FACS) (4),Immunohistochemistry (IHC) (4),Enzyme Immunoassay (EIA) (2),Immunocytochemistry (ICC) (2),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (2),Immunohistochemistry (Frozen Sections) (IHC (fro)) (2),Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) (1)
[Pubmed] 1 reference available
規(guī)格 100 μg
發(fā)貨至 中國 (更改)
發(fā)貨時間 24至29個工作日

Deiodinase, Iodothyronine, Type II (DIO2) (Internal Region) 抗體

產(chǎn)品細(xì)節(jié)

顯示 隱藏
免疫原 Peptide with sequence EVKKHQNQEDRC, from the internal region of the protein sequence according to NP_000784.2, NP_054644.1, NP_001007024.1.
特異性 This antibody is expected to recognise both reported isoform a (NP_000784.2 and NP_054644.1) and isoform b (NP_001007024.1).
交叉反應(yīng) Human, Rat ((Rattus)), Pig (Porcine)
純化方法 Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

目標(biāo)詳細(xì)情況

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Deiodinase, Iodothyronine, Type II (DIO2) (Internal Region) 抗體目標(biāo)詳細(xì)情況

顯示 隱藏
別名 DIO2
背景 Synonyms: DIO2, deiodinase, iodothyronine, type II, 5DII, D2, SelY, TXDI2, thyroxine deiodinase, type II, type 2 iodothyronine deiodinase, type II iodothyronine deiodinaseN: type-II 5?iodinase
基因ID 1734, 13371, 65162
NCBI Accession NP_001007024, NP_054644, NP_000784
研究領(lǐng)域 Cancer, Endocrine system, Hormones

使用細(xì)節(jié)

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使用細(xì)節(jié)

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應(yīng)用備注 ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary experiments gave an approx 65-70 kDa band in Human Heart and Thyroid lysates after 0.05 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.5 kDa according to NP_NP_000784.2 and of 34.6 kDa according to NP_001007024.1. The 65-70 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Cryo-sectioned Mouse Brain (Hippocampus) shows staining in the granular layer of the dentate gyrus. Recommended concentration: 2-3 µg/mL. Data obtained from anonymous customer.
限制 For Research Use only

貯存及處理

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貯存及處理

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狀態(tài) Liquid
緩沖液 Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
儲存液 Sodium azide
Deiodinase, Iodothyronine, Type II (DIO2) (Internal Region) 抗體注意事項(xiàng) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事項(xiàng) Minimize freezing and thawing.
儲存條件 -20 °C
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