TNFSF11抗體 返回列表頁(yè)

TNFSF11抗體

抗原
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11)
別名 TNFSF11, BTF3L1, HUMBTFB, OCIF, OPG, TNFRSF11B, CD254, ODF, OPGL, OPTB2, RANKL, TRANCE, hRANKL2, sOdf, Ly109l, Trance, Opg
適用
其他選擇 Human
Human(115),Murine (103),Rat ((Rattus)) (32),Dog (Canine) (25),Bovine (13),Chicken (1),Pig (Porcine) (1)
宿主
其他選擇 Rabbit
Rabbit(118),Rat (26),Mouse (25),Goat (7),Sheep (1)
克隆類型
多克隆
標(biāo)記
其他選擇 非結(jié)合性
Biotin(23),PE (10),FITC (4),HRP (4),Alexa Fluor 350 (2),Alexa Fluor 488 (2),Alexa Fluor 555 (2),Alexa Fluor 647 (2),Cy3 (2),Cy5 (2),Cy5.5 (2),Cy7 (2),APC (1),Alkaline Phosphatase (AP) (1)
應(yīng)用范圍
其他選擇 Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB)(126),ELISA (70),Flow Cytometry (FACS) (38),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (26),Immunohistochemistry (IHC) (23),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (20),Immunofluorescence (IF) (17),Immunocytochemistry (ICC) (12),Immunohistochemistry (Frozen Sections) (IHC (fro)) (10),Enzyme Immunoassay (EIA) (8),Immunoprecipitation (IP) (8),Blocking Antibody (Inhibition) (5),Immunoassay (IA) (3),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (3),Neutralization (Neut) (3),Blocking Reagent (BR) (1),Functional Studies (Func) (1)
[Pubmed] 2 references available
規(guī)格 0.1 mg
發(fā)貨至 中國(guó) (更改)
發(fā)貨時(shí)間 29至35個(gè)工作日

TNFSF11抗體

產(chǎn)品細(xì)節(jié)

引用 貯存及處理 使用細(xì)節(jié) 目標(biāo)詳細(xì)情況 回頁(yè)首

產(chǎn)品細(xì)節(jié)

顯示 隱藏
免疫原 Highly pure (>98%) recombinant Human sRANKL.
特異性 This antibody reacts with soluble RANK Ligand.
純化方法 Affinity Chromatography.

目標(biāo)詳細(xì)情況

引用 貯存及處理 使用細(xì)節(jié) 回頁(yè)首

目標(biāo)詳細(xì)情況

顯示 隱藏
別名 CD254 / RANKL
背景 RANKL is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. There are three isoforms of RANKL. Human RANKL is a soluble 20 kDa polypeptide, comprising the TNF homologous region of RANKL (176 amino acid residues). This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor associated factor (TRAF) 6, which indicated that this protein may have a role in the regulation of cell apoptosis. RANKL deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis.Synonyms: ODF, OPGL, Osteoclast differentiation factor, Osteoprotegerin ligand, RANK Ligand, RANKL, Receptor activator of nuclear factor kappa B ligand, TNF-related activation-induced cytokine, TNFSF11, TRANCE, Tumor necrosis factor ligand superfamily member 11
基因ID 8600
NCBI Accession NP_003692
UniProt O14788

使用細(xì)節(jié)

引用 貯存及處理 目標(biāo)詳細(xì)情況 回頁(yè)首

使用細(xì)節(jié)

顯示 隱藏
應(yīng)用備注 Neutralization. Immunohistochemistry on Formalin-Fixed, Parrafin-embedded Sections: 0.25?μg/mL withan overnight incubation at 4?°C. Staining of Human metastatic carcinoma of lymph nodefrom breast tissue. An HRP-labeled polymer detection system was used with DABchromogen. Heat induced antigen retrieval with a?pH 6.0 Sodium Citrate buffer isrecommended. Sandwich ELISA: To detect Human sRANKL by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of at least 0.5-2.0?μg/mL of this antibody is required. This antigenaffinity purified antibody, in conjunction with Biotinylated anti-Human sRANKL (PP1086B1or PP1086B2) as a Detection antibody, allows the detection of at least 0.2-0.4?ng/well ofrecombinant Human sRANKL. Western blot: To detect Human sRANKL by Western Blot analysis this antibody can be usedat a concentration of 0.1-0.2?μg/mL. Used in conjunction with compatible secondary
限制 For Research Use only

貯存及處理

引用 使用細(xì)節(jié) 目標(biāo)詳細(xì)情況 回頁(yè)首

貯存及處理

顯示 隱藏
溶解方式 Restore in sterile water to a concentration of 0.1-1.0?mg/mL.
緩沖液 PBS,?pH 7.2 without preservatives
儲(chǔ)存液 Without preservative
注意事項(xiàng) Avoid repeated freezing and thawing.
儲(chǔ)存條件 4 °C/-20 °C
儲(chǔ)存方法 Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

xy-5289R phospho-DOK1(Tyr362)磷酸化D酪氨酸激酶衰減蛋白1抗體
xy-5291R phospho-DAXX (Ser495)磷酸化Fas相關(guān)死亡結(jié)構(gòu)域蛋白抗體
xy-5292R phospho-DAXX (Ser517)磷酸化Fas相關(guān)死亡結(jié)構(gòu)域蛋白抗體
xy-5293R phospho-DAXX (Ser671)磷酸化Fas相關(guān)死亡結(jié)構(gòu)域蛋白抗體
xy-5294R phospho-Dnmt1(Ser154)磷酸化DNA甲基轉(zhuǎn)移酶1抗體
xy-5295R phospho-Dnmt1(Ser732)磷酸化DNA甲基轉(zhuǎn)移酶1抗體
xy-5296R phospho-Dnmt1(Tyr399)磷酸化DNA甲基轉(zhuǎn)移酶1抗體
xy-5057R DHCR77脫氫膽固醇還原酶抗體
xy-8173R Dbx2大腦發(fā)育同源蛋白2抗體
xy-4057R DEGS1退行性精母細(xì)胞同源物1抗體
xy-7720R DSCC1DSCC1蛋白抗體
xy-4962R DcR2誘捕受體2抗體
xy-13009R DMAP1DNA甲基轉(zhuǎn)移酶相關(guān)蛋白1抗體
xy-13010R DMC1減數(shù)分裂同源蛋白DMC1抗體
xy-13011R DNA Ligase IIIDNA連接酶3抗體
xy-13012R phospho-DNA PKcs (Thr2638)磷酸化DNA依賴性蛋白激酶抗體
xy-13013R phospho-DNA PKcs (Thr2647)磷酸化DNA依賴性蛋白激酶抗體
xy-13014R DNA polymerase delta p50DNA聚合酶δ2/DNA pol δ 2抗體
xy-13015R DNA Polymerase delta, catalytic subunitDNA聚合酶δ催化亞單位/DNA pol δ cat抗體
xy-13016R DNA polymerase etaDNA聚合酶η抗體
xy-13017R DNA Polymerase gammaDNA聚合酶γ/DNA pol γ抗體
xy-13018R DNA Polymerase iotaDNA聚合酶ι/DNA pol ι抗體
xy-13019R DNA Polymerase Kappa/POLKDNA聚合酶κ/DNA pol κ抗體
xy-13020R DNA Polymerase lambdaDNA聚合酶λ/DNA pol λ抗體
xy-13021R DNA polymerase muDNA聚合酶μ/DNA pol μ抗體
xy-13023R DNAJA2細(xì)胞周期蛋白3抗體
xy-13024R DNAJC9DNAJC9蛋白抗體
xy-13025R Dnmt2DNA甲基轉(zhuǎn)移酶2抗體