LXN 抗體 返回列表頁

LXN 抗體

抗原
Latexin (LXN)
別名 wu:fk66c02, zgc:103612, LXN, lxn, ECI, TCI
抗原表位
其他選擇 AA 2-222
AA 2-222(22),N-Term (10),Internal Region (9),AA 1-222 (8),C-Term (7),AA 31-60 (6),AA 85-115 (6),AA 200-250 (2),AA 78-107 (2),Center (2),AA 1-223 (1),AA 70-86 (1),Middle Region (1)
適用
其他選擇 Human
Human(80),Murine (43),Rat ((Rattus)) (20),Bovine (13),Pig (Porcine) (12),Dog (Canine) (2)
宿主
其他選擇 Mouse
Rabbit(72),Mouse (24),Goat (6),Chicken (2),Sheep (1)
克隆類型 (克隆位點(diǎn))
單克隆 (2)
標(biāo)記
其他選擇 非結(jié)合性
FITC(9),Biotin (7),PE (5),HRP (4),APC (3),Alkaline Phosphatase (AP) (3),Alexa Fluor 350 (1),Alexa Fluor 488 (1),Alexa Fluor 555 (1),Alexa Fluor 647 (1),Cy3 (1),Cy5 (1),Cy5.5 (1),Cy7 (1)
應(yīng)用范圍
其他選擇 Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Western Blotting (WB)(79),ELISA (55),Immunohistochemistry (IHC) (26),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (23),Flow Cytometry (FACS) (18),Immunocytochemistry (ICC) (11),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (10),Immunofluorescence (IF) (7),Immunohistochemistry (Frozen Sections) (IHC (fro)) (5),Enzyme Immunoassay (EIA) (4),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (4),Immunoprecipitation (IP) (2),Immunoassay (IA) (1),Neutralization (Neut) (1)
規(guī)格 50 μg
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LXN 抗體

產(chǎn)品細(xì)節(jié)

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產(chǎn)品細(xì)節(jié)

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免疫原 Recombinant Human Latexin protein
克隆位點(diǎn) 2
亞型 IgG1
特異性 Human Latexin / Lxn
產(chǎn)品特性 This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, E.coli-derived, recombinant human Latexin (rh Latexin, NP_064554.3, Glu 2-Glu 222). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
純化方法 Affinity Purified
過濾 0.2 μm filtered

目標(biāo)詳細(xì)情況

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目標(biāo)詳細(xì)情況

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別名 LXN
背景 Latexin, also known as tissue or endogenous carboxypeptidase inhibitor (TCI or ECI), is the only known endogenous specific inhibitor of zinc-dependent metallocarboxypeptidases (MCPs) present in mammalians so far. Latexin is originally identified as a molecular marker for the regional specification of the neocortex in development in rats. The 222 amino acid latexin in human shows different expression distribution with high levels in heart, prostate, ovary, kidney, pancreas, and colon, but only moderate or low levels in other tissues including brain. Latexin is also expressed at high levels and is inducible in macrophages in concert with other protease inhibitors and potential protease targets, and thus is suggested to play a role in inflammation and innate immunity pathways. Despite of the non-detectable sequence similarity with plant and parasite inhibitors, Latexin is related to a human putative tumor suppressor protein, TIG1. In addition, Latexin is also implicated in Alzheimer's disease.
NCBI Accession NP_064554.3
研究領(lǐng)域 Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Proteolysis / Ubiquitin, Innate Immunity

使用細(xì)節(jié)

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使用細(xì)節(jié)

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應(yīng)用備注 Flow Cytometry - Human LXN expression in MCF-7 cells. The cells were treated according to manufacturer's manual , and stained with Purified Mouse anti-LXN (10211-MM02, 1 μg/test), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCalibur flow cytometry system.
限制 For Research Use only

貯存及處理

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貯存及處理

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緩沖液 PBS with 5% trehalose
儲存液 Without preservative
注意事項(xiàng) Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
儲存條件 4 °C/-20 °C/-80 °C
儲存方法 This antibody can be stored at 2 °C-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C.
有效期 12 months

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