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當(dāng)前位置:武漢原生原代生物醫(yī)藥科技有限公司>>技術(shù)文章
當(dāng)前位置:武漢原生原代生物醫(yī)藥科技有限公司>>技術(shù)文章
2021
11-152021
11-152021
11-11PriCells: Isolation of human primary airway smooth muscle
PriCells:Isolationofhumanprimaryairwaysmoothmuscle1.Primaryculturesofhumanprimaryairwaysmoothmuscle(...2021
11-11PriCells: Isolation of Human Pulmonary Epithelial Cells
PriCells:IsolationofHumanPulmonaryEpithelialCells1.Pulmonaryepithelialcellsfromhumanlungtissuewereis...2021
11-11PriCells: Isolation and growth of pulmonary artery adventitial fibroblasts
PriCells:Isolationandgrowthofpulmonaryarteryadventitialfibroblasts1.Adventitiafromthemainpulmonaryar...2021
11-11PriCells: Primary Culture of Pulmonary Arterial Smooth Muscle Cells
PriCells:PrimaryCultureofPulmonaryArterialSmoothMuscleCells1.PrimaryculturesofPulmonaryArterialSmoot...2021
11-11PriCells: Measurement of primary endothelial cell permeability to fluxes of dextran or albumin
PriCells:MeasurementofprimaryendothelialcellpermeabilitytofluxesofdextranoralbuminThefluxesofalbumin...2021
11-102021
11-10PriCells: Isolation and Culture of Human Brain Tumor Stem Cell
PriCells:IsolationandCultureofHumanBrainTumorStemCellTheisolation,culture,identification,andpurifica...2021
11-10PriCells: Identification and expansion of the tumorigenic lung cancer stem cell population
PriCells:IdentificationandexpansionofthetumorigeniclungcancerstemcellpopulationLungcancercontainsara...2021
11-10PriCells: Isolation and Culture of Human Liver Stem Cells
PriCells:IsolationandCultureofHumanLiverStemCellsMaterialsandMethods1.Humanhepatocyteswereisolatedfr...2021
11-10PriCells: Isolation and Culture of Multipotent Stem Cells from Human Bone Morrow.
PriCells:IsolationandCultureofMultipotentStemCellsfromHumanBoneMorrow.Bonemarrowcontainsthreetypesof...2021
11-09PriCells: Ex Vivo Human Primary Mesenchymal Stem Cells (MSCs) Culture
PriCells:ExVivoHumanPrimaryMesenchymalStemCells(MSCs)CultureHumanbonemarrowcontainsmesenchymalprogen...2021
11-082021
11-08PriCells: 正常大鼠小膠質(zhì)細胞的培養(yǎng)
PriCells:正常大鼠小膠質(zhì)細胞的培養(yǎng)實驗材料:1.新生大鼠或小鼠;2.不含Ca2+和Mg2+的1×PBS,添加200000IU/L青霉素、200mg/L鏈霉素,pH7.2;3.培養(yǎng)用液:含10%...2021
11-08PriCells: 標(biāo)記的鏈霉卵白素-生物素法(LSAB)
PriCells:標(biāo)記的鏈霉卵白素-生物素法(LSAB)標(biāo)記的鏈霉卵白素-生物素法(LSAB法)是標(biāo)記的卵白素技術(shù),20世紀(jì)80年代開始采用?;驹噭孩俚谝豢贵w;②生物素化第二抗體;③辣根過氧化物酶...2021
11-08PriCells: 免疫酶細胞化學(xué)技術(shù)中的雙PAP法檢測微量抗原
PriCells:免疫酶細胞化學(xué)技術(shù)中的雙PAP法檢測微量抗原在復(fù)合物體系中連接更多的PAP復(fù)合物,來進一步放大抗原,從而使靈敏度更為增強,適用于檢測微量抗原。實驗方法:1.將固定后的標(biāo)本用PBS漂洗...2021
11-08PriCells: 原代細胞培養(yǎng)后過氧化物酶檢測
PriCells:原代細胞培養(yǎng)后過氧化物酶檢測試劑和器材:1.過氧化物儲存液:30mmol/L過氧化氫儲存在T-牛血清白蛋白(Tris-牛血清白蛋白溶液)中;2.樣品緩沖液:2體積的T-牛血清白蛋白溶...2021
11-05PriCells: 原代細胞培養(yǎng)后,碘克沙醇梯度溶液分析主要細胞器
PriCells:原代細胞培養(yǎng)后,碘克沙醇梯度溶液分析主要細胞器試劑和器材:1.OptiPrepTM(600g/L的碘克沙醇);2.勻漿介質(zhì):0.25mmol/L蔗糖溶液、1mmol/L乙二胺四乙酸、...2021
11-05不連續(xù)Nycodenz梯度溶液中原代細胞溶酶體的分離
PriCells:不連續(xù)Nycodenz梯度溶液中原代細胞溶酶體的分離試劑和器材:1.勻漿介質(zhì):0.25mol/L蔗糖溶液、1mmol/L乙二胺四乙酸、10mmol/LHepes-NaOH,pH7.4...請輸入賬號
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